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A comprehensive study of chromosome 16q in invasive ductal and lobular breast carcinoma using array CGH

  • Oncogenomics
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Abstract

We analysed chromosome 16q in 106 breast cancers using tiling-path array-comparative genomic hybridization (aCGH). About 80% of ductal cancers (IDCs) and all lobular cancers (ILCs) lost at least part of 16q. Grade I (GI) IDCs and ILCs often lost the whole chromosome arm. Grade II (GII) and grade III (GIII) IDCs showed less frequent whole-arm loss, but often had complex changes, typically small regions of gain together with larger regions of loss. The boundaries of gains/losses tended to cluster, common sites being 54.5–55.5 Mb and 57.4–58.8 Mb. Overall, the peak frequency of loss (83% cancers) occurred at 61.9–62.9 Mb. We also found several ‘minimal’ regions of loss/gain. However, no mutations in candidate genes (TRADD, CDH5, CDH8 and CDH11) were detected. Cluster analysis based on copy number changes identified a large group of cancers that had lost most of 16q, and two smaller groups (one with few changes, one with a tendency to show copy number gain). Although all morphological types occurred in each cluster group, IDCs (especially GII/GIII) were relatively overrepresented in the smaller groups. Cluster groups were not independently associated with survival. Use of tiling-path aCGH prompted re-evaluation of the hypothetical pathways of breast carcinogenesis. ILCs have the simplest changes on 16q and probably diverge from the IDC lineage close to the stage of 16q loss. Higher-grade IDCs probably develop from low-grade lesions in most cases, but there remains evidence that some GII/GIII IDCs arise without a GI precursor.

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Acknowledgements

We are very grateful to colleagues at the Sanger Institute Microarray Facility for array printing.

Author information

Author notes

  1. J E Watson

    Present address: Amgen Inc., 1120 Veterans Blvd, South San Francisco, CA, 94080, USA

Authors and Affiliations

  1. Molecular and Population Genetics Laboratory, Cancer Research UK, Lincoln's Inn Fields, London, UK

    R Roylance, P Gorman, T Papior, Y-L Wan, M Ives, N Wortham & I Tomlinson

  2. Collins's Lab S151, UCSF Cancer Center, San Francisco, CA, USA

    J E Watson & C Collins

  3. The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

    C Langford, H Fiegler & N Carter

  4. Hedley Atkins/CR-UK Breast Pathology Laboratory, Guy's Hospital, London, UK

    C Gillett

  5. Mathematics, Statistics and Epidemiology, Bart's and the London Medical School, Charterhouse Square, London, UK

    P Sasieni

  6. Department of Histopathology, Addenbrooke's Hospital, Cambridge, UK

    S Pinder

  7. c/o Department of Histopathology, Academic Unit of Pathology, University of Leeds, St James' University Hospital, Leeds, UK

    A Hanby

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  1. R Roylance
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  2. P Gorman
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  3. T Papior
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  4. Y-L Wan
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  6. J E Watson
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  7. C Collins
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  8. N Wortham
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  9. C Langford
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  10. H Fiegler
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  11. N Carter
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  12. C Gillett
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  13. P Sasieni
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  14. S Pinder
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  15. A Hanby
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  16. I Tomlinson
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Correspondence to R Roylance.

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Roylance, R., Gorman, P., Papior, T. et al. A comprehensive study of chromosome 16q in invasive ductal and lobular breast carcinoma using array CGH. Oncogene 25, 6544–6553 (2006). https://doi.org/10.1038/sj.onc.1209659

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  • DOI: https://doi.org/10.1038/sj.onc.1209659

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