Abstract
The molecular mechanisms regulating the cell fate decision between self-renewal and differentiation/apoptosis in stem and progenitor cells are poorly understood. Here, we report the first comprehensive identification of genes potentially involved in the switch from self-renewal toward differentiation of primary, non-immortalized erythroid avian progenitor cells (T2EC cells). We used the Serial Analysis of Gene Expression (SAGE) technique in order to identify and quantify the genome fraction functionally active in a self-renewing versus a differentiating cell population. We generated two SAGE libraries and sequenced a total of 37 589 tags, thereby obtaining the first transcriptional profile characterization of a chicken cell. Tag identification was performed using a new relational database (Identitag) developed in the laboratory, which allowed a highly satisfactory level of identification. Among 123 differentially expressed genes, 11 were investigated further and for nine of them the differential expression was subsequently confirmed by real-time PCR. The comparison of tag abundance between the two libraries revealed that only a small fraction of transcripts was differentially expressed. The analysis of their functions argue against a prominent role for a master switch in T2EC cells decision-making, but are in favor of a critical role for coordinated small variations in a relatively small number of genes that can lead to essential cellular identity changes.
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Acknowledgements
We thank the SIGENAE group (especially Patrice Deshais, Christophe Klopp and Madeleine Douaire) for sharing chicken EST clusters sequences. This work was supported by the CNRS, UCBL, INRA (‘AGENA’ Program), Région Rhône-Alpes (‘Génomique fonctionnelle des récepteurs d’hormone’ Program), Fondation de France, Association pour la Recherche contre le Cancer and a joint INSERM/AFM program (‘Cellules souches’ Program). Sequencing was partially funded through an InterEPST program (‘Séquençage à grande échelle’, call 2002). FD is a fellow of the EU RTN ‘Hematopoiesis’ program (contract HPRN-CT-2000-00083). SGG is a fellow of the Région Rhône-Alpes. SD is a fellow of the Ligue contre le Cancer and the Association pour la Recherche contre le Cancer.
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Damiola, F., Keime, C., Gonin-Giraud, S. et al. Global transcription analysis of immature avian erythrocytic progenitors: from self-renewal to differentiation. Oncogene 23, 7628–7643 (2004). https://doi.org/10.1038/sj.onc.1208061
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DOI: https://doi.org/10.1038/sj.onc.1208061
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