Skip to main content
SpringerLink
Log in

High throughput fluorescent CE-SSCP SNP genotyping

  • Immediate Communication
  • Published:
Molecular Psychiatry Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Large numbers of single nucleotide polymorphisms (SNPs) are being identified by several laboratories for the purpose of developing dense genetic maps. Single-strand conformation polymorphism (SSCP) analysis has been widely used as a method for detecting novel sequence variations in PCR products. Differences in migration of single-stranded DNA can be used not only to find mutations, but to genotype SNPs in large sample populations. Using PCR with fluorescent labeling and automated capillary electrophoresis SSCP (CE-SSCP), we have developed a panel of 15 functional candidate SNPs. With an automated single capillary instrument, relatively rapid and low cost CE-SSCP SNP genotyping using currently available technology is feasible for 135 000 genotypes per year. With parallel multiple array capillary electrophoresis, more genotypes per year may be attainable.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Psychiatry, Laboratory for Developmental Neuroscience, Child and Adolescent Psychiatry, University of Chicago, MC3077, 5841 S Maryland Avenue, Chicago, 60637, Illinois, USA

    D Gonen, J Veenstra-VanderWeele, Z Yang, B L Leventhal & E H Cook Jr

  2. Department of Pediatrics, University of Chicago, MC3077, 5841 S Maryland Avenue, Chicago, 60637, Illinois, USA

    B L Leventhal & E H Cook Jr

Authors
  1. D Gonen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J Veenstra-VanderWeele
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Z Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B L Leventhal
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E H Cook Jr
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E H Cook Jr.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gonen, D., Veenstra-VanderWeele, J., Yang, Z. et al. High throughput fluorescent CE-SSCP SNP genotyping. Mol Psychiatry 4, 339–343 (1999). https://doi.org/10.1038/sj.mp.4000564

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.mp.4000564

  • Springer Nature Limited

Keywords

This article is cited by

Search

Navigation