Thermomyces lanuginosus
was subjected to three cycles of mutagenesis (UV/NTG) and a selection procedure to develop amylase-hyperproducing, catabolite-repression-resistant and partially constitutive strains. One of the selected derepressed mutant strain III51, produced ∼7- and 3-fold higher specific activity of α-amylase (190 U/mg protein) and glucoamylase (105 U/mg protein), respectively, compared to a wild-type parental strain. Further, the effect of production parameters on mutant strain III51 was studied using a Box–Behnken design. The regression models computed showed significantly high R 2 values of 96 and 97% for α-amylase and glucoamylase activities, respectively, indicating that they are appropriate for predicting relationships between corn flour, soybean meal and pH with α-amylase and glucoamylase production. Journal of Industrial Microbiology & Biotechnology (2002) 29, 70–74 doi:10.1038/sj.jim.7000270
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Received 05 July 2001/ Accepted in revised form 16 April 2002
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Rubinder, K., Chadha, B., Singh, N. et al. Amylase hyperproduction by deregulated mutants of the thermophilic fungus Thermomyces lanuginosus . J Ind Microbiol Biotech 29, 70–74 (2002). https://doi.org/10.1038/sj.jim.7000270
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DOI: https://doi.org/10.1038/sj.jim.7000270