Zymograms of a crude protein extract from S. cerevisiae strain SCPP containing endopolygalacturonase were studied and compared to the purified enzyme by determining their physico-chemical properties. The results obtained with crude extract were similar to those of the purified enzyme. The endopolygalacturonase from both sources displayed a pH optimum between 3.0 and 4.0, and was active at temperatures between 4 and 50°C on a large panel of substrates. These characteristics make this S. cerevisiae endopolygalacturonase an attractive tool for the beverage industry. Journal of Industrial Microbiology & Biotechnology (2000) 24, 296–300.
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Received 24 September 1999/ Accepted in revised form 29 January 2000
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Belarbi, A., Hachet, C., Helfer, AC. et al. Study of some physico-chemical characteristics of a Saccharomyces cerevisiae endopolygalacturonase: a possible use in beverage industry. J Ind Microbiol Biotech 24, 296–300 (2000). https://doi.org/10.1038/sj.jim.2900820
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DOI: https://doi.org/10.1038/sj.jim.2900820