Measurement of potential activity of fixed nitrifying bacteria in biological filters used in drinking water production

Nitrification during biological filtration is being used more and more in drinking water production to remove ammonia, which can be the source of several water quality problems during distribution. In this process, ammonia is converted into nitrite and then into nitrate by fixed autotrophic nitrifying bacteria. The purpose of this work was to develop a technique to estimate fixed nitrifying biomass (sum of ammonia- and nitrite-oxidizing populations). The quantification of autotrophic nitrifying biomass was determined by potential nitrifying activity measurement. The production of oxidized forms of inorganic nitrogen (nitrates and nitrites) was measured after an incubation of 2 cm³ of colonized solid support in the presence of a 5-ml nitrifier medium containing 10 mg N-NH₄ L⁻¹ for 30 min at 32°C. The production rate of oxidized nitrogen in optimal conditions was measured and converted into nitrifying biomass by using the maximum specific oxidizing activity. This technique was shown to be appropriate for conditions encountered in the biological filters used in drinking water production and sufficiently simple to be used for routine measurements. Journal of Industrial Microbiology & Biotechnology (2000) 24, 161–166.