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A series of laboratory exercises utilizing luxR gene of Vibrio fischeri and gfp gene of Aequoria victoria to teach the broad applications of polymerase chain reaction

  • J A Slock
  • P M Farrugia
  • T A Horn
  • J A Phillips
  • J T Smedley
  • M J Romanko
  • 355 Downloads

Three experiments are described; directional cloning of the luxR gene from the bioluminescent marine bacterium, Vibrio fischeri, directional cloning of the gfpgene from the marine jelly fish, Aequoria victoria, and the construction of a LuxR-GFP fusion protein. Experiments are presented using lux and gfp in an undergraduate biology curriculum. Journal of Industrial Microbiology & Biotechnology (2000) 24, 345–352.

Keywords: polymerase chain reaction (PCR); lux bioluminescence; green fluorescence protein (GFP); directional cloning; fusion protein 

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Copyright information

© Society for Industrial Microbiology 2000

Authors and Affiliations

  • J A Slock
    • 1
  • P M Farrugia
    • 1
  • T A Horn
    • 1
  • J A Phillips
    • 1
  • J T Smedley
    • 1
  • M J Romanko
    • 1
  1. 1.King’s College, Department of Biology, Wilkes-Barre, PA 18711, USAUS

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