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A new xylanase from a Trichoderma harzianum strain

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Journal of Industrial Microbiology and Biotechnology

A new xylanase (XYL2) was purified from solid-state cultures of Trichoderma harzianum strain C by ultrafiltration and gel filtration. SDS-PAGE of the xylanase showed an apparent homogeneity and molecular weight of 18 kDa. It had the highest activity at pH 5.0 and 45°C and was stable at 50°C and pH 5.0 up to 4 h xylanase. XYL2 had a low K m with insoluble oat spelt xylan as substrate. Compared to the amino acid composition of xylanases from Trichoderma spp, xylanase XYL2 presented a high content of glutamate/glutamine, phenylalanine and cysteine, and a low content of serine. Xylanase XYL2 improved the delignification and selectivity of unbleached hardwood kraft pulp.

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Authors and Affiliations

  1. Laboratório de Enzimologia, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, CEP 70910-900, Brazil, , , , , , BR

    F Q de Paula Silveira & E X F Filho

  2. CBSP-Centro Brasileiro de Serviços e Pesquisas em Proteínas, Departmento de Biologia Celular, Universidade de Brasília, DF, CEP 70910-900, Brazil, , , , , , BR

    M V de Sousa & C A O Ricart

  3. Faculdade de Engenharia Química de Lorena, Departamento de Biotecnologia, Caixa Postal 116, Lorena, SP, CEP 12600-000, Brazil, , , , , , BR

    A M F Milagres & C L de Medeiros

Authors
  1. F Q de Paula Silveira
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  2. M V de Sousa
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  3. C A O Ricart
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  4. A M F Milagres
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  5. C L de Medeiros
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  6. E X F Filho
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Additional information

Received 02 February 1999/ Accepted in revised form 17 April 1999

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de Paula Silveira, F., de Sousa, M., Ricart, C. et al. A new xylanase from a Trichoderma harzianum strain. J Ind Microbiol Biotech 23, 682–685 (1999). https://doi.org/10.1038/sj.jim.2900682

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  • DOI: https://doi.org/10.1038/sj.jim.2900682

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