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Molecular detection of bacterial indicators in cosmetic/pharmaceuticals and raw materials

  • L Jimenez
  • R Ignar
  • S Smalls
  • P Grech
  • J Hamilton
  • Y Bosko
  • D English

PCR assays were compared with standard microbiological methods for rapid detection of the United States Pharmacopoeia (USP) bacterial indicators in artificially contaminated samples of raw materials and cosmetic/pharmaceutical products. DNA primers containing the specific sequences of the uidA gene of the β-glucuronidase enzyme for Escherichia coli, the membrane lipoprotein gene oprL for Pseudomonas aeruginosa, and the 16S ribosomal gene for Staphylococcus aureus were used for detection in the PCR reaction. Contaminated samples were incubated for 24 h at 35°C. After incubation in broth media with and without 4% Tween 20, samples were streaked on selective growth media. After 5–6 days, all microbial indicators were morphologically and biochemically identified using standard methods while detection and identification by the PCR-based assays was completed within 27–30 h. Rapid PCR detection of E. coli, S. aureus, and P. aeruginosa will allow a faster quality evaluation and release of raw materials and cosmetic/pharmaceutical products sensitive to microbial contamination.

Keywords: PCR; USP; bacterial indicators; rapid detection; cosmetic/pharmaceuticals; quality control; Staphylococcus aureus; Escherichia coli; Pseudomonas aeruginosa 

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Copyright information

© Society for Industrial Microbiology 1999

Authors and Affiliations

  • L Jimenez
    • 1
  • R Ignar
    • 1
  • S Smalls
    • 1
  • P Grech
    • 1
  • J Hamilton
    • 1
  • Y Bosko
    • 1
  • D English
    • 1
  1. 1.Microbiology Laboratory, Research and Development, Block Drug Company, Jersey City, New Jersey 07302, USAUS

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