Abstract
OBJECTIVE: To develop a monoclonal antibody that recognises an epitope of the native β3-adrenoceptor expressed on the extracellular surface of human cells and tissues.
DESIGN: A high affinity monoclonal antibody, Mab72c, was raised against the human β3-adrenoceptor expressed on a transfected mammalian cell line.
RESULTS: In CHO (Chinese hamster ovary) cells transfected with β3-adrenoceptor cDNA, antibody labelling was found to be proportional to receptor density measured by the binding of the radiolabelled β-adrenoceptor antagonist, [125I]-iodocyanopindolol. The use of Mab 72c has demonstrated the expression of the β3-adrenoceptor in a variety of human tissues, including gall bladder, prostate and colon, where a mRNA signal had been detected previously. This study also provides the first direct demonstration of the expression of β3-adrenoceptors in human skeletal muscle, atrium and adipose tissue.
CONCLUSION: The development of this antibody represents an important addition to the armentarium of reagents that are available to study the localisation of β3-adrenoceptors in human tissues.
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Chamberlain, P., Jennings, K., Paul, F. et al. The tissue distribution of the human β3-adrenoceptor studied using a monoclonal antibody: Direct evidence of the β3-adrenoceptor in human adipose tissue, atrium and skeletal muscle. Int J Obes 23, 1057–1065 (1999). https://doi.org/10.1038/sj.ijo.0801039
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DOI: https://doi.org/10.1038/sj.ijo.0801039
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