Abstract
An organ culture model of rabbit corpus cavernosum has been developed to investigate fibrillar collagen synthesis in intact organ. Rabbit peni were removed en bloc, the corpora cavernosa were dissected, sliced into 5 mm pieces and incubated in culture media up to 24 h. Tissue viability and metabolic and functional integrity were assessed by (a) lactate dehydrogenase (LDH) release, (b) protein synthesis, and (c) ability to respond to exogenously added cytokines.
Explants, maintained for 24 h in organ culture, exhibited minimal LDH release and maintained functional integrity determined from protein synthesis and ability to synthesize collagen in response to TGF-β1. These data suggest that explants of rabbit corpus cavernosum in organ culture represent a viable model to investigate connective tissue protein biosynthesis in vitro.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Newhall, P., Traish, A. & Moreland, R. Development of an organ culture experimental system to investigate collagen synthesis in corpora cavernosa. Int J Impot Res 10, 77–81 (1998). https://doi.org/10.1038/sj.ijir.3900309
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.ijir.3900309
- Springer Nature Limited
Keywords
This article is cited by
-
Phenotypic transition of corpus cavernosum smooth muscle cells subjected to hypoxia
Cell and Tissue Research (2014)
-
Role of oxidative stress and antioxidants in Peyronie's disease
International Journal of Impotence Research (2002)