Abstract
Transduction of dendritic cells (DC) can result in presentation of tumor-associated antigens and induction of immunity against undefined epitopes. The present studies demonstrate adenovirus (Ad)-mediated transduction of the β-galactosidase gene in mouse DC. Similar transductions have been obtained with the gene encoding the DF3/MUC1 tumor-associated antigen. We show that the Ad-transduced DC are functional in primary allogeneic mixed lymphocyte reactions. Mice immunized with Ad-transduced DC develop cytotoxic T lymphocytes that are specific for the β-galactosidase or DF3/MUC1 antigens. The results also demonstrate that Ad.MUC1-transduced DC induce a specific response which inhibits the growth of DF3/MUC1- positive tumors. These findings support the usefulness of Ad-transduced DC for in vivo immunization against tumor-associated antigens.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gong, J., Chen, L., Chen, D. et al. Induction of antigen-specific antitumor immunity with adenovirus-transduced dendritic cells. Gene Ther 4, 1023–1028 (1997). https://doi.org/10.1038/sj.gt.3300496
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/sj.gt.3300496
- Springer Nature Limited
Keywords
This article is cited by
-
Induction of protective and therapeutic anti-pancreatic cancer immunity using a reconstructed MUC1 DNA vaccine
BMC Cancer (2009)
-
Engineered lentivector targeting of dendritic cells for in vivo immunization
Nature Biotechnology (2008)
-
Specific targeting of whole lymphoma cells to dendritic cells ex vivo provides a potent antitumor vaccine
Journal of Translational Medicine (2007)
-
Mannan-modified adenovirus as a vaccine to induce antitumor immunity
Gene Therapy (2007)
-
Dendritic Cell Function After Gene Transfer with Adenovirus-calcium Phosphate Co-precipitates
Molecular Therapy (2007)