Skip to main content
SpringerLink
Log in

Allografting

The implication of follicular lymphoma patients receiving allogeneic stem cell transplantation from donors carrying t(14;18)-positive cells

  • Original Article
  • Published:
Bone Marrow Transplantation Submit manuscript

Summary:

We performed real-time quantitative polymerase chain reaction (RQ-PCR) in peripheral blood (PB) and/or bone marrow (BM) samples collected pre- and post transplant from 23 recipient–donor pairs receiving allogeneic stem cell transplantation (allo-SCT) for follicular lymphoma (FL). Of 23 donors, 11 had a PB and/or BM sample positive for t(14;18) (BCL2/IGH fusion) at low levels (<one t(14;18) cell in 10K total cells). Recipients from donors with (n=11) and those without (n=12) detectable t(14:18) cells were similar in age, sex, and disease status pretransplant. No differences in the incidence of graft-versus-host-disease (GVHD), delayed engraftment, relapse rate, disease-free survival and overall survival were identified between the groups. Two recipients without detectable t(14;18) cells pre-transplant showed detectable t(14;18) cells at 2 and 11 years after receiving grafts from donors with t(14:18) cells. Neither patient developed FL 1.5 and 2 years after the emergence of t(14;18) cells. Although the sample size is relatively small, our findings suggest that individuals carrying t(14;18) cells may not be excluded as donors given the lack of an association of t(14;18) detected in donors with adverse clinical outcome. It may be necessary to screen for the donor's t(14;18) status before using t(14;18) for monitoring minimal residual disease by RQ-PCR to exclude the possibility of confounding donor's t(14;18) clone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1

Similar content being viewed by others

References

  1. Turner GE, Ross FM, Krajewski AS . Detection of t(14;18) in British follicular lymphoma using cytogenetics, Southern blotting and the polymerase chain reaction. Br J Haematol 1995; 89: 223–225.

    Article  CAS  Google Scholar 

  2. Pappa VI, Wilkes S, Salam A et al. Use of the polymerase chain reaction and direct sequencing analysis to detect cells with the t(14;18) in autologous bone marrow from patients with follicular lymphoma, before and after in vitro treatment. Bone Marrow Transplant 1998; 22: 553–558.

    Article  CAS  Google Scholar 

  3. Aster JC, Longtine JA . Detection of BCL2 rearrangements in follicular lymphoma. Am J Pathol 2002; 160: 759–763.

    Article  CAS  Google Scholar 

  4. Yasukawa M, Bando S, Dolken G et al. Low frequency of BCL-2/J(H) translocation in peripheral blood lymphocytes of healthy Japanese individuals. Blood 2001; 98: 486–488.

    Article  CAS  Google Scholar 

  5. Ladetto M, Drandi D, Compagno M et al. PCR-detectable nonneoplastic Bcl-2/IgH rearrangements are common in normal subjects and cancer patients at diagnosis but rare in subjects treated with chemotherapy. J Clin Oncol 2003; 21: 1398–1403.

    Article  CAS  Google Scholar 

  6. Ji W, Qu GZ, Ye P et al. Frequent detection of bcl-2/JH translocations in human blood and organ samples by a quantitative polymerase chain reaction assay. Cancer Res 1995; 55: 2876–2882.

    CAS  Google Scholar 

  7. Summers KE, Goff LK, Wilson AG et al. Frequency of the Bcl-2/IgH rearrangement in normal individuals: implications for the monitoring of disease in patients with follicular lymphoma. J Clin Oncol 2001; 19: 420–424.

    Article  CAS  Google Scholar 

  8. Tsimberidou AM, Jiang Y, Ford RJ et al. Quantitative real-time polymerase chain reaction for detection of circulating cells with t(14;18) in volunteer blood donors and patients with follicular lymphoma. Leuk Lymphoma 2002; 43: 1589–1598.

    Article  CAS  Google Scholar 

  9. Fuscoe JC, Setzer RW, Collard DD, Moore MM . Quantification of t(14;18) in the lymphocytes of healthy adult humans as a possible biomarker for environmental exposures to carcinogens. Carcinogenesis 1996; 17: 1013–1020.

    Article  CAS  Google Scholar 

  10. Liu Y, Hernandez AM, Shibata D, Cortopassi GA . BCL2 translocation frequency rises with age in humans. Proc Natl Acad Sci USA 1994; 91: 8910–8914.

    Article  CAS  Google Scholar 

  11. Dolken G, Illerhaus G, Hirt C, Mertelsmann R . BCL-2/JH rearrangements in circulating B cells of healthy blood donors and patients with nonmalignant diseases. J Clin Oncol 1996; 14: 1333–1344.

    Article  CAS  Google Scholar 

  12. Finke J . The role of stem cell transplantation in the treatment of follicular lymphoma. Semin Cancer Biol 2003; 13: 233–239.

    Article  Google Scholar 

  13. van Besien K, Loberiza Jr FR, Bajorunaite R et al. Comparison of autologous and allogeneic hematopoietic stem cell transplantation for follicular lymphoma. Blood 2003; 102: 3521–3529.

    Article  CAS  Google Scholar 

  14. Juckett M, Rowlings P, Hessner M et al. T cell-depleted allogeneic bone marrow transplantation for high-risk non-Hodgkin's lymphoma: clinical and molecular follow-up. Bone Marrow Transplant 1998; 21: 893–899.

    Article  CAS  Google Scholar 

  15. Stein RS, Greer JP, Goodman S et al. High-dose therapy with autologous or allogeneic transplantation as salvage therapy for small cleaved cell lymphoma of follicular center cell origin. Bone Marrow Transplant 1999; 23: 227–233.

    Article  CAS  Google Scholar 

  16. Chang CC, Bredeson C, Juckett M et al. Tumor load in patients with follicular lymphoma post stem cell transplantation may correlate with clinical course. Bone Marrow Transplant 2003; 32: 287–291.

    Article  Google Scholar 

  17. Rosenblum MD, Drobyski WR, Keever-Taylor C, Chang CC . Concurrent presence of both patient and donor t(14;18) in a follicular lymphoma patient after undergoing allogeneic BMT: implications for minimal residual disease detection post-transplant. Bone Marrow Transplant 2003; 31: 947–949.

    Article  CAS  Google Scholar 

  18. Dolken L, Schuler F, Dolken G . Quantitative detection of t(14;18)-positive cells by real-time quantitative PCR using fluorogenic probes. Biotechniques 1998; 25: 1058–1064.

    Article  CAS  Google Scholar 

  19. Hirt C, Dolken G . Quantitative detection of t(14;18)-positive cells in patients with follicular lymphoma before and after autologous bone marrow transplantation. Bone Marrow Transplant 2000; 25: 419–426.

    Article  CAS  Google Scholar 

  20. Ladetto M, Sametti S, Donovan JW et al. A validated real-time quantitative PCR approach shows a correlation between tumor burden and successful ex vivo purging in follicular lymphoma patients. Exp Hematol 2001; 29: 183–193.

    Article  CAS  Google Scholar 

  21. Mandigers CM, Meijerink JP, Mensink EJ et al. Lack of correlation between numbers of circulating t(14;18)-positive cells and response to first-line treatment in follicular lymphoma. Blood 2001; 98: 940–944.

    Article  CAS  Google Scholar 

  22. Keever-Taylor CA, Craig A, Molter M et al. Complement-mediated T-cell depletion of bone marrow: comparison of T10B9.1A-31 and Muromonab-Orthoclone OKT3. Cytotherapy 2001; 3: 467–481.

    Article  CAS  Google Scholar 

  23. Viardot A, Barth TF, Moller P et al. Cytogenetic evolution of follicular lymphoma. Semin Cancer Biol 2003; 13: 183–190.

    Article  Google Scholar 

  24. Caporaso N, Marti GE, Goldin L . Perspectives on familial chronic lymphocytic leukemia: genes and the environment. Semin Hematol 2004; 41: 201–206.

    Article  CAS  Google Scholar 

  25. Mandigers CM, Meijerink JP, Raemaekers JM et al. Graft-versus-lymphoma effect of donor leucocyte infusion shown by real-time quantitative PCR analysis of t(14;18). Lancet 1998; 352: 1522–1523.

    Article  CAS  Google Scholar 

  26. Sarris AH, Jiang Y, Tsimberidou AM et al. Quantitative real-time polymerase chain reaction for monitoring minimal residual disease in patients with advanced indolent lymphomas treated with rituximab, fludarabine, mitoxantrone, and dexamethasone. Semin Oncol 2002; 29 (1 Suppl. 2): 48–55.

    Article  CAS  Google Scholar 

  27. Summers KE, Davies AJ, Matthews J et al. The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction. Br J Haematol 2002; 118: 563–566.

    Article  CAS  Google Scholar 

  28. Jenner MJ, Summers KE, Norton AJ et al. JH probe real-time quantitative polymerase chain reaction assay for Bcl-2/IgH rearrangements. Br J Haematol 2002; 118: 550–558.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, The Methodist Hospital, Baylor College of Medicine, Houston, TX, USA

    D K McGregor

  2. Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA

    C A Keever-Taylor, C Bredeson & D H Vesole

  3. Department of Pathology, Medical College of Wisconsin, Milwaukee, WI, USA

    B Schur

  4. Department of Biostatistics, Medical College of Wisconsin, Milwaukee, WI, USA

    B Logan

  5. Department of Pathology, The Methodist Hospital, Weil Medical College, Cornell University, Houston, TX, USA

    C-C Chang

Authors
  1. D K McGregor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C A Keever-Taylor
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C Bredeson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B Schur
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D H Vesole
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B Logan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. C-C Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C-C Chang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

McGregor, D., Keever-Taylor, C., Bredeson, C. et al. The implication of follicular lymphoma patients receiving allogeneic stem cell transplantation from donors carrying t(14;18)-positive cells. Bone Marrow Transplant 35, 1049–1054 (2005). https://doi.org/10.1038/sj.bmt.1704969

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.bmt.1704969

  • Springer Nature Limited

Keywords

This article is cited by

Search

Navigation