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Autografting

Association of post-thaw viable CD34+ cells and CFU-GM with time to hematopoietic engraftment

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Summary:

In all, 78 peripheral hematopoietic progenitor cell collections from 52 patients were evaluated using our previously published validated post-thaw assays at the time of collection and following transplantation by assessment of viable CD34+ cells, and granulocyte–macrophage colony-forming units (CFU-GM) cryopreserved in quality control vials. The median (range) post-thaw recovery of viable CD34+ cells and CFU-GM was 66.4% (36.1–93.6%) and 63.0% (28.6–85.7%), respectively, which did not show significant correlation with the engraftment of either neutrophils (P=0.136 and 0.417, respectively) or platelets (P=0.88 and 0.126, respectively). However, the reinfused viable CD34+ cells/kg of patient weight pre- or post-cryopreservation showed significant correlation to engraftment of neutrophils (P=0.0001 and 0.001, respectively) and platelets (P=0.023 and 0.010, respectively), whereas CFU-GM pre- or post-cryopreservation was significantly correlated to neutrophils (P=0.011 and 0.007, respectively) but not to platelets (P=0.112 and 0.100, respectively). The results show that post-cryopreservation assessment of viable CD34+ cells or CFU-GM is as reliable a predictor of rapid engraftment as that of pre-cryopreservation measures. Therefore, the post-cryopreservation number of viable CD34+ cells or CFU-GM should be used to eliminate the risks of unforeseen cell loss that could occur during cryopreservation or long-term storage.

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Acknowledgements

This work was supported by Grant XE00013 from the Canadian Blood Services Research & Development program.

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Authors and Affiliations

  1. Canadian Blood Services, Edmonton, Alberta, Canada

    H Yang, J P Acker, M Cabuhat, B Letcher, L Larratt & L E McGann

  2. Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada

    J P Acker & L E McGann

  3. Division of Clinical Hematology, University of Alberta, Edmonton, Alberta, Canada

    L Larratt

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  1. H Yang
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  2. J P Acker
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  3. M Cabuhat
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  4. B Letcher
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  6. L E McGann
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Correspondence to H Yang.

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Yang, H., Acker, J., Cabuhat, M. et al. Association of post-thaw viable CD34+ cells and CFU-GM with time to hematopoietic engraftment. Bone Marrow Transplant 35, 881–887 (2005). https://doi.org/10.1038/sj.bmt.1704926

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  • DOI: https://doi.org/10.1038/sj.bmt.1704926

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