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Molecular farming of antimicrobial peptides

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Antimicrobial peptides have the potential to combat the growing threat of antimicrobial resistance; however, their clinical translation remains challenging. Here, we discuss molecular farming as a sustainable approach to antimicrobial peptide production, outlining different platforms to produce antimicrobial peptides using plants and viral vectors.

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Fig. 1: Antimicrobial peptide production using plant-based platforms.

References

  1. Hancock, R. E. & Sahl, H. G. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat. Biotechnol. 24, 1551–1557.

  2. Chaudhary, S. et al. Efficient in planta production of amidated antimicrobial peptides that are active against drug-resistant ESKAPE pathogens. Nat. Commun. 14, 1464 (2023).

    Article  Google Scholar 

  3. Zeitler, B. et al. Production of a de-novo designed antimicrobial peptide in Nicotiana benthamiana. Plant Mol. Biol. 81, 259–272 (2013).

    Article  Google Scholar 

  4. Marillonnet, S., Thoeringer, C., Kandzia, R., Klimyuk, V. & Gleba, Y. Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat. Biotechnol. 23, 718–723 (2005).

    Article  Google Scholar 

  5. Sainsbury, F., Thuenemann, E. C. & Lomonossoff, G. P. pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants. Plant Biotechnol. J. 7, 682–693 (2009).

    Article  Google Scholar 

  6. O’Keefe, B. R. et al. Engineering soya bean seeds as a scalable platform to produce cyanovirin-N, a non-ARV microbicide against HIV. Plant Biotechnol. J. 13, 884–892 (2015).

    Article  Google Scholar 

  7. Lee, S. B., Li, B., Jin, S. & Daniell, H. Expression and characterization of antimicrobial peptides Retrocyclin-101 and Protegrin-1 in chloroplasts to control viral and bacterial infections. Plant Biotechnol. J. 9, 100–115 (2011).

    Article  Google Scholar 

  8. Wang, Y. P. et al. Chloroplast-expressed MSI-99 in tobacco improves disease resistance and displays inhibitory effect against rice blast fungus. Int. J. Mol. Sci. 16, 4628–4641 (2015).

    Article  Google Scholar 

  9. Nadal, A. et al. Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness. BMC Plant Biol. 12, 159 (2012).

    Article  Google Scholar 

  10. Hashemi, S., Niazi, A., Baghizadeh, A. & Taghizadeh, M. S. Successful use of Nicotiana tabacum hairy roots for the recombinant production of Cecropin A peptide. Biotechnol. Appl. Biochem. 69, 876–886 (2022).

    Article  Google Scholar 

  11. Bardor, M. et al. Immunoreactivity in mammals of two typical plant glyco-epitopes, core alpha(1,3)-fucose and core xylose. Glycobiology 13, 427–434 (2003).

    Article  Google Scholar 

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Acknowledgements

We would like to thank members of the genome engineering and synthetic biology laboratory at the King Abdullah University of Science and Technology (KAUST) for insightful discussions.

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M.M.M. conceived the concept and outline for the article; S.C. and M.M.M. wrote the article.

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Correspondence to Magdy M. Mahfouz.

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The authors declare no competing interests.

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Chaudhary, S., Mahfouz, M.M. Molecular farming of antimicrobial peptides. Nat Rev Bioeng 2, 3–5 (2024). https://doi.org/10.1038/s44222-023-00149-y

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