Metalloprotein activity can be tuned by altering first- and second-sphere interactions with the metal ion or ions. Here, a non-canonical haem axial ligand is introduced into a myoglobin variant, modulating both. The resulting enhancement of cyclopropanation activity illustrates the utility of expanding the suite of available amino acids for biocatalyst engineering.
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Edwards, E.H., Bren, K.L. Carbene capture in a myoglobin mutant. Nat Catal 1, 565–566 (2018). https://doi.org/10.1038/s41929-018-0129-y
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DOI: https://doi.org/10.1038/s41929-018-0129-y
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