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GENETICS

Crispants take the spotlight

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A new CRISPR/Cas9 method that can generate F0 mutant zebrafish has the potential to cut costs, spare time, and reduce animal use for researchers interested in screening loss-of-function alleles in vivo.

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Fig. 1: Kroll et al.’s F0 knockout strategy involves three synthetic guide RNAs, increasing the odds of generating fully penetrant mutants that can be used to study gene function.

References

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  1. Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-Biocenter 1, 1030, Vienna, Austria

    Wouter Masselink

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Masselink, W. Crispants take the spotlight. Lab Anim 50, 95–96 (2021). https://doi.org/10.1038/s41684-021-00739-6

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