Abstract
Recent concepts suggest that both Plasmodium falciparum factors and coagulation contribute to endothelial activation and dysfunction in pediatric cerebral malaria (CM) pathology. However, there is still limited understanding of how these complex inflammatory stimuli are integrated by brain endothelial cells. In this study, we examined how mature-stage P. falciparum infected erythrocytes (IE) interact with tumor necrosis factor α (TNFα) and thrombin in the activation and permeability of primary human brain microvascular endothelial cell (HBMEC) monolayers. Whereas trophozoite-stage P. falciparum-IE have limited effect on the viability of HBMEC or the secretion of pro-inflammatory cytokines or chemokines, except at super physiological parasite-host cell ratios, schizont-stage P. falciparum-IE induced low levels of cell death. Additionally, schizont-stage parasites were more barrier disruptive than trophozoite-stage P. falciparum-IE and prolonged thrombin-induced barrier disruption in both resting and TNFα-activated HBMEC monolayers. These results provide evidence that parasite products and thrombin may interact to increase brain endothelial permeability.
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Introduction
Cerebral malaria (CM) is a life-threatening complication associated with the sequestration of Plasmodium falciparum-infected erythrocytes (IE) in the brain microcirculation1,2,3,4. In African children, CM is associated with endothelial activation and dysfunction5. Recent neuroimaging studies have implicated breakdown of blood brain barrier6 and severe brain swelling7 in disease etiology. The vascular dysfunction in cerebral malaria is believed to result from a combination of microvascular obstruction and tissue perfusion abnormalities8,9, altered coagulation10,11, systemic and local inflammatory processes12, and damaging parasite products (reviewed in Miller et al.13). From in vitro studies, numerous parasite products can activate endothelial cells or increase permeability, including P. falciparum histones14, malaria hemozoin pigment with parasite DNA15, and histidine-rich protein 2 (PfHRP2)16.
Recent findings suggest that coagulation may play an important role in pediatric CM pathogenesis. Pediatric CM autopsies have revealed fibrin deposition5,11, endothelial tissue factor expression17, and platelet recruitment18,19 in cerebral microvessels. Moreover, clinical laboratory evidence of disseminated intravascular coagulation is linked to a higher fatality risk10. Thrombin is a key enzyme in the coagulation cascade and a barrier permeability mediator20. Thrombin converts fibrinogen into fibrin and increases vascular permeability by cleaving protease activated receptor 1 (PAR-1) on endothelial cells20 leading to actin stress fiber formation and disassembly of cell junction proteins that open temporary gaps between cells21. Thrombin’s pro-inflammatory activities are countered by the activated protein C (APC) pathway22. Protein C is a serum protein that binds to endothelial protein C receptor (EPCR) on the endothelial surface and becomes activated by the thrombin-thrombomodulin complex23. APC counteracts thrombin signaling by inhibiting its activation and by upregulating anti-inflammatory, anti-apoptotic, and barrier restorative signals in endothelial cells22. Inflammation shifts the hemostatic mechanisms in favor of thrombosis, and impaired thrombin regulation is implicated in endothelial dysfunction from sepsis and other inflammatory diseases24.
Several findings suggest that the APC pathway is compromised in CM (reviewed in Bernabeu et al.25) due to a combination of loss of EPCR on microvascular endothelial cells11 and parasite interference of the APC-EPCR interaction26. Parasite sequestration is mediated by specific interactions between members of the clonally variant var gene/P. falciparum erythrocyte membrane protein 1 (PfEMP1) family and receptors on the host vascular endothelium27,28,29. EPCR-binding parasites have affinity for human brain endothelial cells in vitro30,31 and are increased in cerebral malaria patients26,32,33,34. Moreover, EPCR-binding var transcripts are linked to severe brain swelling in CM35 and recombinant parasite domains compete with APC for binding to EPCR26,36,37,38,39. Collectively, these findings strongly implicate both parasite factors and pro-coagulant processes in cerebral malaria pathology, although the molecular mechanisms of how these complex inflammatory stimuli translate into endothelial activation and blood-brain barrier dysfunction remain only partially understood.
In this study, we investigated the interaction of mature P. falciparum-IE (trophozoite and schizont stages) and thrombin in co-culture models with primary human brain microvascular endothelial cells (HBMEC). Our study suggests that mature-stage parasites differ in their barrier-disruptive activity and that schizont-stage P. falciparum-IE delay endothelial cell recovery from thrombin-induced barrier disruption.
Results
Comparative evaluation of immortalized and primary brain endothelial cells for thrombin-induced barrier permeability assays
To investigate the interaction of P. falciparum-IE and thrombin in brain endothelial activation and permeability, we first compared surface expression levels of parasite cytoadhesion receptors and the main thrombin receptor PAR-1 on an immortalized and primary brain endothelial cells. Compared to an immortalized brain endothelial cell line (THBMEC40), resting HBMEC expressed higher levels of EPCR (MFI 6041 versus 761), higher levels of PAR-1 (MFI 365 versus 126), and lower levels of ICAM-1 (MFI 29 versus 391) (Supplementary Fig. S1A). In addition, HBMEC were 100% reactive for the endothelial marker, CD31, and upregulated ICAM-1 following TNFα stimulation (MFI 2206) (Supplementary Fig. S1A).
We then compared THBMEC and primary HBMEC for thrombin-induced barrier disruption by xCELLigence Real-Time Cell Analysis (Supplementary Fig. S1B). Following seeding at a similar cell density, both cell types reached a confluency plateau by approximately 18–20 hrs. However, the raw cell index (CI), a quantitative measure of the number of cells, was lower for THBMEC than HBMEC (Supplementary Fig. S1B), likely due to THBMEC having a longer and thinner cell shape. In the xCELLigence assay, relative changes in cell morphology or cell adhesion following treatment with barrier permeability mediators are detected as a reduction of CI. As expected, primary HBMEC responded to 10 nM thrombin treatment with the characteristic rapid decrease in CI that reached a maximum by 15–20 min and returned to baseline by 1 hr. By comparison, thrombin induced a small and extremely truncated (~1 min) decline in CI in THBMEC (Supplementary Fig. S1B, lower right corner), likely due to the very low PAR-1 surface expression in the immortalized brain cells, as low and high doses of thrombin elicit opposing barrier strengthening or barrier disruptive responses in endothelial cells41. Consequently, primary HBMEC were used for the remainder of experiments.
Next, to be able to study whether parasite factors modify thrombin-induced permeability, we identified a concentration of thrombin that would induce a partial barrier disruption. We compared serum-free or 5% serum medium assay conditions because serum contains anti-thrombin factors. We observed a dose-dependent change in CI when comparing 2.5, 5 or 10 nM of thrombin in both conditions (Supplementary Fig. S1C) and choose 5 nM for the remainder of experiments.
TNFα modifies expression levels of EPCR, ICAM-1, and PAR-1 in primary HBMEC
Because pro-inflammatory cytokines can modify the surface levels of cell adhesion molecules and receptors regulating coagulation42,43, we performed a TNFα time course stimulation assay with primary HBMEC (Fig. 1 and Supplementary Fig. S2). The endothelial marker CD31 was expressed by nearly all resting cells and did not change significantly with TNFα stimulation (Fig. 1). As expected, ICAM-1 and VCAM-1 expression levels were highly upregulated by TNFα with slightly delayed kinetics for VCAM-1. By 24 hrs after TNFα stimulation, ICAM-1 surface levels were approximately 50 times higher and the percentage of positive cells had increased from 10% to 60%. Conversely, other known parasite binding partners, including CD3644, gC1qR145, E-selectin (CD62E)46, and fractalkine (CX3CL1)47 were expressed at negligible levels on HBMEC cells, and ICAM-248 levels fluctuated following TNFα treatment (Fig. 1). Of the receptors involved in thrombin signaling, thrombomodulin was expressed by few HBMEC, consistent with previous reports37, and EPCR and PAR-1 surface levels initially decreased and then rebounded (Fig. 1). Since the expression levels were dynamic following TNFα activation, we chose to pre-stimulate HBMEC for 24 hrs, at which point ICAM-1 was highly upregulated and PAR-1 and EPCR expression were nearly equivalent to resting baseline levels.
P. falciparum-IE induce minimal apoptosis of HBMEC
We designed our experiments to simultaneously measure endothelium viability, cellular cytokine response, and permeability from the same HBMEC cultures under different environmental conditions (Fig. 2). To investigate the impact of P. falciparum-IE on HBMEC viability, we compared different parasite binding variants at two development stages. In particular, we compared an EPCR-binding parasite line (IT4var19), a dual EPCR and ICAM-1 binder (HB3var03), and a dual CD36 and ICAM-1 binding parasite line (ItG-ICAM-1)30,49,50,51 at the trophozoite-stage for either a short term (4–6 hrs) or long-term culture (12–20 hrs) or at the schizont-stage IE for a short term (4–6 hrs) (Fig. 2).
HBMEC viability was assessed by annexin-V and propidium iodide (PI) staining at two different cell ratios of trophozoite-stage IE (8–10:1 or 80–120:1 IE:HBMEC ratio) (Fig. 3A). For positive control, the kinase inhibitor staurosporine caused a significant percentage of apoptotic cells (34 ± 5% after 1 hr at 10 μM or 47 ± 8% after 12 hrs at 0.5 μM) (Fig. 3A–C). In short term co-cultures, trophozoite-stage parasite lines induced minimal cell death (3–5%), compared to nE controls (2 ± 0.5%) (Fig. 3B). In overnight cultures, there was a parasite dose-dependent increase in endothelial cell death (9–10%) (Fig. 3C). Similarly, schizont-stage IE (20:1 IE:HBMEC ratio) induced a low level of cell death (5–10%) of both resting or TNFα-activated HBMEC, although the difference did not reach statistical significance (Fig. 3D). Taken together, this analysis suggests that late-stage IE may cause low levels of cell death in both resting and TNFα stimulated HBMEC.
P. falciparum-IE do not induce major pro-inflammatory cytokine release from HBMEC
Previous work suggests that P. falciparum can stimulate dermal and lung endothelial cells to release IL-6, IL-8, and MCP-152,53,54. To investigate whether P. falciparum-IE can trigger pro-inflammatory cytokine or chemokine production from primary HBMEC, supernatants were collected from the trophozoite-stage IE-HBMEC co-cultures at the end of the short-term (4–6 hrs) and long-term (12–20 hrs) incubations. TNFα stimulated production of IL-6, IL-8, and MCP-1 from HBMEC at 24 hrs was used as positive control (Fig. 4). Compared to nE, neither IT4var19 nor ItG-ICAM-1 trophozoite-stage IE induced secretion of IL-6 or IL-8 from HBMEC. However, HB3var03-HBMEC co-cultures had a small but significant secretion of IL-6 comparable to TNFα stimulation (Fig. 4A). Additionally, the chemokine MCP-1 was constitutively released by HBMEC and its secretion was similar or reduced in the presence of either nE or P. falciparum-IE (Fig. 4C).
Interaction of trophozoite-stage IE and thrombin on HBMEC barrier properties
Leukocyte engagement and cross-linking of ICAM-1 on endothelial cells enhances permeability55,56,57,58. To study whether P. falciparum-IE may also increase barrier permeability by this mechanism, we compared parasite lines with low and high ICAM-1 binding activity (HB3var03 and ItG-ICAM-1, respectively)51 to a non-ICAM-1 binder (IT4var19). Using the xCELLigence assay (Supplementary Fig. S3) we found that in resting HBMEC switched to serum-free assay conditions 2 hrs prior to parasite addition (Fig. 5A schematic), all three trophozoite-stage parasite lines induced a statistically significant and dose-dependent reduction in CI values, compared to control nE (Fig. 5B,C). Of the three parasite lines, the high ICAM-1 binding activity ItG-ICAM-1 parasite line was the most barrier disruptive on TNFα stimulated HBMEC (Fig. 5A–C). The same trend was apparent at both the low and high parasite densities, although the difference was statistically significant only at the lower IE-HBMEC ratio (Fig. 5B,C). Of interest, the endothelial barrier properties recovered more rapidly in TNFα-stimulated HBMEC than resting cells for all three parasite lines (Fig. 5A).
We also observed an influence of serum conditions with higher parasite-induced barrier disruption of resting HBMEC that were maintained in serum-supplemented media (+5% FBS) compared to serum-free medium (compare left and right recordings Fig. 5A). However, in the presence of serum, there was no difference between nE and P. falciparum-IE in barrier disruption (Fig. 5). By comparison, the extent of barrier disruption in activated HBMEC was similar in the serum-free and 5% serum conditions (compare left and right recordings Fig. 5A).
To study whether trophozoite-stage-IE modify thrombin-induced barrier permeability, 5 nM thrombin was added two hrs after P. falciparum-IE. Thrombin induced a characteristic rapid loss of barrier properties followed by a recovery in approximately 60–90 mins (Fig. 6A). In both resting and TNFα activated cells, thrombin was additive to the earlier disruption observed with parasites, so the total disruption was larger in monolayers receiving the higher parasite density (Fig. 6B,C). However, the magnitude of the thrombin-induced barrier disruption and the kinetics of barrier recovery were not altered by trophozoite-stage IE. Although thrombin was less disruptive on activated than resting HBMEC monolayers (Fig. 6), the percentage of PAR-1 positive HBMEC was lower at 24 hrs after TNFα stimulation (Fig. 1). As expected, thrombin was less barrier disruptive in the presence of 5% serum (Fig. 6B,C), likely due to anti-thrombin factors in serum. However, as with the serum-free assay conditions, trophozoite-stage IE did not increase the magnitude of thrombin-induced barrier disruption or delay the recovery.
Interaction of schizont-stage IE and thrombin on HBMEC vascular permeability
Previous work suggests that schizonts release bioactive products during red blood cell rupture that activate endothelial cells and increase permeability14,15,16. To investigate whether late stage parasite products interact with thrombin in endothelial permeability, we incubated HBMEC with enriched schizont-stage-IE (1–10:1 ratio, IE:HBMEC). On their own, P. falciparum-IE induced a rapid CI decrease in HBMEC that was sustained over the next 4 hrs (Fig. 7A,B). The magnitude of the parasite-induced CI decrease was similar on resting and TNFα activated cells (Fig. 7A,B). Notably, schizont-stage IE induced equivalent barrier disruption to trophozoite-stage IE at a 10-fold lower IE:HBMEC ratio (compare Figs 5 and 7).
To study whether schizont-stage parasites modify thrombin-induced permeability, 5 nM thrombin was added two hrs after P. falciparum-IE. The effect of thrombin was additive to the earlier disruption observed with schizont-stage IE (Fig. 7A–C). However, in contrast to trophozoite-stage IE, thrombin-induced barrier recovery was delayed, as indicated by a slower slope of recovery compared to nE (Fig. 7C). The delay in barrier recovery following thrombin treatment was observed in both resting and TNFα-activated HBMEC (Fig. 7C), indicating that schizont-stage IE prolong thrombin-induced barrier disruption.
Discussion
During infection, endothelial cells integrate cues from a variety of pro-inflammatory and coagulation factors to effectively respond to pathogens. Overstimulation of endothelial cells in response to inflammation can lead to maladapted microvascular environments24. From pediatric CM autopsy studies, sequestered IE, thrombin and/or fibrin are sometimes co-localized in cerebral microvessels5,59. However, little is known about how parasite factors and host inflammatory products may interact to cause endothelial dysfunction in CM. The aim of our present study was to investigate the interaction of P. falciparum-IE and thrombin on resting and activated primary human brain endothelial cells.
In vitro models have suggested that P. falciparum-IE can activate endothelial cell monolayers to secrete pro-inflammatory cytokines52,53,54,60, induce apoptosis60,61,62,63,64, or increase vascular permeability16,62,65. However, much of this analysis has been conducted on immortalized brain endothelial cell models16,61,62,63,64 and less work has been done on primary brain microvascular endothelial cells65,66. Multiple mechanisms have been proposed by which P. falciparum-IE may activate endothelial cells including pathways at early time points following parasite adhesion, such as release of IE-derived extracellular vesicles containing functional miRNA-Argonaute 2 complexes67 or cross-linking of adhesion receptors68, or later events associated with rupture of IE14,16. Here, we compared trophozoite or schizont-stage IE in co-cultures with primary HBMEC. Whereas P. falciparum-IE can induce IL-6, IL-8, and MCP-1 in primary human dermal and lung microvascular endothelial cells14,69, we observed a very low and dose-dependent release of IL-6 in primary HBMEC to only one of the three parasite lines. The difference in findings could relate to endothelial cell type differences or parasite-dependencies, as cytokine and chemokine secretion was also a variable phenotype for different parasite isolates co-cultured with dermal endothelial cells69. Of interest, there was also a relatively low cell death in primary HBMEC compared to previous work on immortalized brain endothelial cells showing much higher cell death61,62 and increased death following TNFα activation64. There are several possible explanations for these discrepancies, including that the susceptibility to apoptosis has been found to differ between primary pulmonary endothelial cells and immortalized brain endothelial cells61,63. Moreover, the cell death inducing phenotype differs between parasite isolates61,63,70,71 and is influenced by the buffering capacity of culture media63. Thus, both assay conditions and cell and parasite models may influence the endothelial cell death phenotype.
Our main finding relates to the interaction between parasites and thrombin in HBMEC barrier disruption. Thrombin’s activity is under tight regulation with multiple feedback mechanisms to control thrombosis. Although previous work has revealed cross-talk between inflammatory cytokines and thrombosis24, there has been little investigation into whether parasites factors interact with thrombin to exacerbate endothelial dysfunction. Using primary HBMEC, we show that schizont-stage parasites cause higher barrier disruption than trophozoite-stage parasites, even at 10-fold lower parasite concentrations. Of interest, the extent of parasite-induced barrier disruption was influenced by serum assay conditions. Further work is needed to more fully understand how serum withdrawal may have interacted with parasites to induce vascular permeability in HBMEC. However, serum contains growth factors, and serum withdrawal can trigger stress responses in cells, including the induction of reactive oxygen species response within 3–4 hrs72,73. Unlike trophozoites, schizont-stage parasites interacted to prolong thrombin-induced barrier disruption, and thus may act like pro-inflammatory cytokines that have previously been shown to extend thrombin-induced barrier disruption in endothelial cells74. Although the underlying mechanism(s) of the interaction remain to be determined, these findings have important implications for disease mechanisms in cerebral malaria pathogenesis, since both thrombin and sequestered IE are frequently co-localized in pediatric CM brain autopsy specimens59. Thus, this finding raises the possibility that barrier-perturbing factors released by late stage P. falciparum-IE may interact with thrombin to amplify endothelial dysfunction in cerebral malaria. A limitation of this work is that it was performed on endothelial monolayers, which differ from intact microvessels75. Nevertheless, significant molecular insights into barrier regulation have been gained from endothelial monolayer models21.
Taken together, our findings support a model (Fig. 8) in which sequestration of early stage trophozoite IE causes minimal endothelial cell death or permeability on their own, especially at lower parasitemia. By comparison, sequestered late-stage IE may increase localized endothelial dysfunction, by releasing barrier disruptive factors that interact with thrombin in increasing barrier permeability. Moreover, when sequestered IE engage EPCR on brain endothelial cells this may further imbalance microvascular hemostasis by impairing the cytoprotective and barrier restorative APC-EPCR interaction that normally counteracts thrombin and other inflammatory stimuli26,37,38,39. In summary, these findings suggest potential interactions between thrombin and sequestered parasites in endothelial dysfunction.
Methods
Human microvascular endothelial cell cultures
Transformed brain endothelial cells (THBMEC), provided by Dr. Monique Stins (Johns Hopkins Bloomberg School of Public Health), were used from passage 38 to 39 and cultured as previously reported30,40,76. Primary human brain microvascular endothelial cells (HBMEC, ACBRI376) were purchased from Cell Systems at passage 3. Cells were expanded in Endothelial basal medium (EBM) in accordance with manufacturer specifications on an extracellular matrix-coated surface (Attachment Factor, Cell Systems) and frozen at passage 5. Cells were used in experiments from passage 5 to 12 (average 8–9) in Endothelial Cell Growth Medium MV2 (EGM-2 MV Bulletkit) with 5% FBS and supplements provided by PromoCell. HBMEC were certified positive by the manufacturer for expression of Von Willebrand factor, for acetylated low-density lipoprotein uptake, and for CD31 expression. HBMEC were routinely monitored for CD31 by flow cytometry. All cells tested negative for mycoplasma.
Surface receptor labelling of human brain endothelial cells
THBMEC or HBMEC cells were seeded on collagen coated flasks and cultured for three to four days until confluent. Cells were rinsed with HBSS and then lifted with 15 mM EDTA in HBSS. Then 5 × 104 cells were distributed into wells of a 96 well plate and washed 1X with PBS (0.5% BSA) at room temperature. Antibody labelling was performed on ice for 30 min each step, using the following: goat anti-human CX3CL1/Fractalkine (15 μg/ml, R&D Systems, AF365) or goat anti-human EPCR (30 μg/ml, R&D Systems, AF2245) followed by rabbit anti-goat Alexa488 coupled antibodies (A-21467, Molecular Probes, 1/400). Mouse anti-human VCAM-1/CD106 (10 μl for 105 cells, R&D Systems, FAB5649P) followed by goat anti-mouse Alexa488 coupled antibodies (Molecular Probes, 1/200). Mouse anti-human CD31 (BD Pharmingen, mAb, 560983, clone WM59) or mouse anti-human ICAM-1 (0.5 μg/ml, Abcam, ab19756) were detected by PE-labelled mouse monoclonal IgG1 (2 μl for 105 cells, Abcam, ab91357). Directly conjugated Alexa488 or FITC mouse monoclonal isotype control IgG1 (2 μg/ml, Abcam, ab106163) were used to control for labeling by mouse anti-human PAR-1 Alexa488-conjugated antibody (0.5 μl for 105 cells, R&D systems, FAB3855G), mouse anti-human CD36 FITC-conjugated antibody (10 μl for 105 cells, Abcam, ab39022), mouse anti-human ICAM-2 (2 μg/ml, Abcam, ab47207), mouse anti-human thrombomodulin FITC-conjugated antibody (1 μl for 105 cells, Abcam, ab27396), and mouse anti-human gC1qR FITC-conjugated antibody (1/50, Abcam, ab125138). For assays with proinflammatory pre-stimulation, confluent cell monolayers were stimulated with 10 ng/ml TNFα (Sigma, T0157) for 24 hrs at 37 °C. For the TNFα time course experiment, HBMEC were analyzed at 1, 2, 4, 8, 12, 18 and 24 hrs. Results were expressed relative to control (no treatment) and the percentage of positive gated cells was reported. Prior to analysis in an LSRII (Becton Dickinson), cells were fixed with 2% w/v of paraformaldehyde for 10 min and 15,000 single live cells were gated using the Live/Dead fixable violet Dead cell stain kit (Molecular Probes, L34955). Data was analyzed using FLOWJO v10 software (Tree Star Inc.).
Apoptosis assays
Mature trophozoite-stage or schizont-stage enriched IE cultures were incubated with HBMEC monolayers for 4–6 or 12–20 hrs intervals at ratios ranging from 8–12 (106) to 80–120 (107) IE per endothelial cell. As a control, 10 μM or 0.5 μM staurosporine (Abcam, ab120056) was added for 1 or 4 hrs to trigger HBMEC apoptosis. For assays with TNFα pre-stimulation, cells were stimulated with 10 ng/ml TNFα (Sigma, T0157) for 24 hrs at 37 °C. For flow analysis, cells were lifted with trypsin/EDTA solution which also led to the detachment of most of the bound IE. We then labeled with Annexin V and PI according to manufacturer’s specifications (Abcam, ab14085). As red blood cells are much smaller than endothelial cells, the following cytometer settings (FSC 130 and SSC 150) were applied to gate only for the endothelial cells. Relative Annexin V/PI fluorescence was measured by flow cytometry on a LSRII cell sorter (Becton Dickinson) and analyzed using FLOWJO V10 software (Tree Star Inc.).
Production of cytokines by HBMEC
The supernatants from wells of IE:HBMEC co-cultures used above for the apoptosis assays were harvested before the wash steps and stored at −20 °C. Cytokine levels were quantified by sandwich ELISA kits for human IL-6 (ThermoFisher, 88–7066), IL-8 (CXCL8) (ThermoFisher, 88–8086) and MCP-1 (CCL2) (ThermoFisher, 88–7399) following the manufacturer’s instructions. The culture supernatants were diluted in a final volume of 0.1 ml as follows: 1/2 for IL-6, 1/40 for IL-8 and 1/40 for MCP-1, and their respective concentration in pg/ml was determined using standard curves. For positive control, the level of cytokine secretion by HBMEC was measured following pre-stimulation with 10 ng/ml TNFα for 24 hrs. For negative control, supernatants from co-incubation with either media alone or normal O+ erythrocytes (nE O+) were used.
Parasite lines
P. falciparum parasites were cultured using human O+ erythrocytes in the presence of 10% pooled human A+ serum (Valley BioMedical, US) under standard conditions77. The ItG-ICAM-1 parasite line78, IT4var19 parasite line50 and HB3var03 parasite line31 were synchronized by alternating sorbitol treatment and gelatin selection. Mature trophozoite-stage IE (16–36 hrs post-invasion) or schizont-stage IE (>40 hrs and segmented) were enriched via magnet-column purification before being incubated with endothelial cell monolayers for 4–6 hrs or 12–20 hrs (overnight) at ratios ranging from 8–12 (106) to 80–120 (107) IE per endothelial cell. All parasite lines were regularly screened for mycoplasma negativity by PCR-based VenorGeM detection kit (Sigma, MP0025).
Permeability assays
Endothelial permeability assays were measured using xCELLigence system from ACEA Biosciences. THBMEC and primary HBMEC were grown in EGM-2 (5% FBS) for 3–4 days to reach confluence in 96 well plates on integrated electronic sensors. The xCELLigence instrument measures real time changes in transendothelial resistance (TER) by electric cell-substrate impedance sensing (ECIS). For thrombin-induced barrier permeability assays, the media covering the cell monolayers was first changed to the experimental media condition (EGM-2 with 5% FBS or serum-free EGM-2 media complemented with all growth supplements) for 2 hrs, followed by the addition of the experimental concentration of IE or nE O+ suspension for 2 hrs. For thrombin challenge, 5 nM thrombin (T6884, Sigma) was added 2 hrs after IE addition to HBMEC. Control wells received nE O+ alone and/or thrombin alone. To evaluate the barrier restoration kinetics, the ECIS measurements were recorded for 2–3 hrs following thrombin treatment. We expressed the activity of thrombin-induced permeability to the maximum disruption observed as the minimum Cell Index (CI) and calculated the Area Under the Curve (AUC) for each given recorded period. The best-fit slope (m) and goodness of fit (R2) values were calculated from the recovery phase starting (dash line) corresponding to the maximum disruption time point observed.
Statistical analyses
Statistical analysis was performed using Prism (version 7, GraphPad Software Inc.). Apoptosis data were compared using one-way ANOVA, Kruskal-Wallis non-parametric t test corrected by method of Benjamini, Krieger and Yekutieli. ELISA data were analyzed by the Mann-Whitney non-parametric unpaired, one-tailed t test. Permeability assays were compared using one-way ANOVA, Kruskal-Wallis non-parametric t test followed by Dunn’s Test for multiple comparisons. For each condition, nE was used as control.
Data Availability
All data generated or analyzed during this study are included in the published article (and its Supplementary Information Files).
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Funding for this work was provided by the National Institutes of Health (RO1 AI114766).
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Concept development: M.A. Coordinated the study: M.A. & J.D.S. Experimental work: M.A., M.B. & M.-M.D. Data analysis: M.A., M.B. & M.-M.D. Wrote the paper: M.A. & J.D.S. All authors reviewed the manuscript.
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Avril, M., Benjamin, M., Dols, MM. et al. Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption. Sci Rep 9, 13142 (2019). https://doi.org/10.1038/s41598-019-49530-1
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DOI: https://doi.org/10.1038/s41598-019-49530-1
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