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A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96

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Fig. 1: Overview of the optoConfig-96 workflow.

Data availability

The image data used to generate the correction matrices for the 24-well plate adapter are available upon request. The calculated correction matrices are available online at https://www.github.com/WeberSynBioLab/optoConfig-96.

Code availability

optoConfig-96 is available at https://www.github.com/WeberSynBioLab/optoConfig-96, along with the most up-to-date version of the user guide (Supplementary Manual).

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Acknowledgements

We thank J. Schmidt, D. Renz and L. Breh (technical workshop of the Faculty of Biology, University of Freiburg, Germany) for assembling the optoPlates-96 and 3D printing of the 24-well plate adapter. We also thank A. Fischer, C. Jerez-Longres, L. Lataster, H. Mohsenin and N. Urban for testing optoConfig-96. This work was supported in part by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy CIBSS–EXC-2189, Project ID 390939984. This work was supported in part by the Excellence Initiative of the Deutsche Forschungsgemeinschaft (GSC–4, Spemann Graduate School) and in part by the Ministry for Science, Research and Arts of the State of Baden-Wuerttemberg.

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Authors and Affiliations

  1. Faculty of Biology, University of Freiburg, Freiburg, Germany

    Oliver S. Thomas, Maximilian Hörner & Wilfried Weber

  2. Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany

    Oliver S. Thomas, Maximilian Hörner & Wilfried Weber

  3. Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany

    Oliver S. Thomas & Wilfried Weber

Authors
  1. Oliver S. Thomas
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  2. Maximilian Hörner
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  3. Wilfried Weber
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Contributions

O.S.T., M.H. and W.W. conceptualized optoConfig-96. O.S.T. wrote the software. M.H. designed and characterized the 24-well plate adapter. M.H. and W.W. supervised the project. O.S.T., M.H. and W.W. wrote and edited the manuscript.

Corresponding authors

Correspondence to Maximilian Hörner or Wilfried Weber.

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The authors declare no competing interests.

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Peer review information Nature Protocols thanks Andreas Möglich and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Supplementary information

Supplementary Information

Supplementary Notes 1 and 2, Supplementary Manual and Supplementary References

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Thomas, O.S., Hörner, M. & Weber, W. A graphical user interface to design high-throughput optogenetic experiments with the optoPlate-96. Nat Protoc 15, 2785–2787 (2020). https://doi.org/10.1038/s41596-020-0349-x

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  • DOI: https://doi.org/10.1038/s41596-020-0349-x

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