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Cryo-EM structure reveals how SID-1 recognizes dsRNA that initiates systemic RNAi

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Systemic RNA interference (RNAi) in Caenorhabditis elegans is initiated by SID-1-mediated double-stranded RNA (dsRNA) internalization. By combining cryo-electron microscopy (cryo-EM), in vitro and in vivo assays, we show how SID-1 specifically recognizes dsRNA and provide important insights into dsRNA internalization by SID-1.

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Fig. 1: Electron microscopy structures of SID-1 homologs and the SID-1–dsRNA complexes.

References

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This is a summary of: Zhang, J. et al. Structural insights into double-stranded RNA recognition and transport by SID-1. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-024-01276-9 (2024).

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Cryo-EM structure reveals how SID-1 recognizes dsRNA that initiates systemic RNAi. Nat Struct Mol Biol (2024). https://doi.org/10.1038/s41594-024-01277-8

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  • DOI: https://doi.org/10.1038/s41594-024-01277-8

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