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DNA REPLICATION AND REPAIR

The partner-swapping sliding clamp loader exposed

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Eukaryotes possess several clamp loaders comprising four common subunits and a fifth subunit unique to each complex. The RFC-A–E clamp loader loads the PCNA clamp at 3′-recessed structures for DNA replication. However, swapping a single subunit, Rad24, for RFC-A yields a clamp loader that prefers the 911 clamp and 5′-recessed DNA. Three new studies reveal detailed views of the clamp loading reaction and provide insights into substrate preferences of each loader.

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Fig. 1: Sliding clamps and clamp loaders.
Fig. 2: The DNA replication and damage-response clamp loaders.

References

  1. Yao, N. Y. & O’Donnell, M. E. Subcell. Biochem. 96, 233–258 (2020).

    Article  Google Scholar 

  2. Bell, S. P. & Labib, K. Genetics 203, 1027–1067 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Kelch, B. A. Biopolymers 105, 532–546 (2016).

    Article  CAS  PubMed  Google Scholar 

  4. Bowman, G. D., Goedken, E. R., Kazmirski, S. L., O’Donnell, M. & Kuriyan, J. FEBS Lett. 579, 863–867 (2005).

    Article  CAS  PubMed  Google Scholar 

  5. Lee, K. & Park, S. H. Exp. Mol. Med. 52, 1948–1958 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Majka, J. & Burgers, P. M. J. Prog. Nucleic Acid Res. 78, 227–260 (2004).

    Article  CAS  Google Scholar 

  7. Choe, K. N. & Moldovan, G.-L. Mol. Cell 65, 380–392 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Attali, I., Botchan, M. R. & Berger, J. M. S. Annu. Rev. Biochem. 90, 1–30 (2021).

    Article  Google Scholar 

  9. Zheng, F., Georgescu, R. E., Yao, N. Y., O’Donnell, M. E. & Li, H. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-022-00742-6 (2022).

    Article  PubMed  Google Scholar 

  10. Castaneda, J. C., Schrecker, M., Remus, D. & Hite, R. K. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-022-00741-7 (2022).

    Article  PubMed  Google Scholar 

  11. Gaubitz, C. et al. eLife 11, e74175 (2022).

    Article  PubMed  PubMed Central  Google Scholar 

  12. Parrilla-Castellar, E. R., Arlander, S. J. H. & Karnitz, L. DNA Rep. 3, 1009–1014 (2004).

    Article  CAS  Google Scholar 

  13. Khan, Y. A., White, K. I. & Brunger, A. T. Crit. Rev. Biochem. Mol. https://doi.org/10.1080/10409238.2021.1979460 (2021).

    Article  Google Scholar 

  14. Arbel, M., Choudhary, K., Tfilin, O. & Kupiec, M. Genes (Basel) 12, 1812 (2021).

    Article  CAS  Google Scholar 

  15. Ellison, V. & Stillman, B. PLoS Biol. 1, e33 (2003).

    Article  PubMed  PubMed Central  Google Scholar 

  16. Majka, J., Binz, S. K., Wold, M. S. & Burgers, P. M. J. J. Biol. Chem. 281, 27855–27861 (2006).

    Article  CAS  PubMed  Google Scholar 

  17. Zou, L., Liu, D. & Elledge, S. J. Proc. Natl Acad. Sci. USA 100, 13827–13832 (2003).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Yoshida, K. & Fujita, M. Cell. Mol. Life Sci. 78, 6763–6773 (2021).

    Article  CAS  PubMed  Google Scholar 

  19. Lanz, M. C., Dibitetto, D. & Smolka, M. B. EMBO J. 38, e101801 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

  20. Waterman, D. P., Haber, J. E. & Smolka, M. B. Annu. Rev. Biochem. 89, 103–133 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Kelch, B. A., Makino, D. L., O’Donnell, M. & Kuriyan, J. Science 334, 1675–1680 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Jeruzalmi, D., O’Donnell, M. & Kuriyan, J. Cell 106, 429–441 (2001).

    Article  CAS  PubMed  Google Scholar 

  23. Jeruzalmi, D. et al. Cell 106, 417–428 (2001).

    Article  CAS  PubMed  Google Scholar 

  24. Bowman, G. D., O’Donnell, M. & Kuriyan, J. Nature 429, 724–730 (2004).

    Article  CAS  PubMed  Google Scholar 

  25. Simonetta, K. R. et al. Cell 137, 659–671 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, City College of New York, New York, NY, USA

    David Jeruzalmi

  2. PhD Program in Biochemistry, The Graduate Center of the City University of New York, New York, NY, USA

    David Jeruzalmi

  3. PhD Program in Biology, The Graduate Center of the City University of New York, New York, NY, USA

    David Jeruzalmi

  4. PhD Program in Chemistry, The Graduate Center of the City University of New York, New York, NY, USA

    David Jeruzalmi

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  1. David Jeruzalmi
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Correspondence to David Jeruzalmi.

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Jeruzalmi, D. The partner-swapping sliding clamp loader exposed. Nat Struct Mol Biol 29, 283–286 (2022). https://doi.org/10.1038/s41594-022-00761-3

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