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Stretching the resolution limit of atomic force microscopy

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Atomic force microscopy (AFM) is unique in visualizing functional biomolecules in aqueous solution at ~1 nm resolution. By borrowing localization methods from fluorescence microscopy, AFM has been shown to discern structural domains that may be separated by only a few Ångströms.

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Fig. 1: Factors that can limit spatial resolution in bio-AFM.
Fig. 2: From peaks to peaking probability and LAFM.

References

  1. Walker, M. L. et al. Nature 405, 804–807 (2000).

    Article  CAS  Google Scholar 

  2. Yildiz, A. et al. Science 300, 2061–2065 (2003).

    Article  CAS  Google Scholar 

  3. Kodera, N., Yamamoto, D., Ishikawa, R. & Ando, T. Nature 468, 72–76 (2010).

    Article  CAS  Google Scholar 

  4. Heath, G. R. et al. Nature 594, 385–390 (2021).

    Article  CAS  Google Scholar 

  5. Fukuma, T., Kobayashi, K., Matsushige, K. & Yamada, H. Appl. Phys. Lett. 87, 034101 (2005).

    Article  Google Scholar 

  6. Hoogenboom, B. W. et al. Appl. Phys. Lett. 88, 193109 (2006).

    Article  Google Scholar 

  7. Engel, A. & Müller, D. J. Nat. Struct. Biol. 7, 715–718 (2000).

    Article  CAS  Google Scholar 

  8. Schabert, F. A. & Engel, A. Biophys. J. 67, 2394–2403 (1994).

    Article  CAS  Google Scholar 

  9. Asakawa, H. et al. Biophys. J. 101, 1270–1276 (2011).

    Article  CAS  Google Scholar 

  10. Ido, S. et al. ACS Nano 7, 1817–1822 (2013).

    Article  CAS  Google Scholar 

  11. Dufrêne, Y. F. et al. Nat. Nanotechnol. 12, 295–307 (2017).

    Article  Google Scholar 

  12. Gan, Y. Surf. Sci. Rep. 64, 99–121 (2009).

    Article  CAS  Google Scholar 

  13. Odin, C., Aimé, J. P., El Kaakour, Z. & Bouhacina, T. Surf. Sci. 317, 321–340 (1994).

    Article  CAS  Google Scholar 

  14. Fechner, P. et al. Biophys. J. 96, 3822–3831 (2009).

    Article  CAS  Google Scholar 

  15. Scheuring, S., Müller, D. J., Stahlberg, H., Engel, H.-A. & Engel, A. Eur. Biophys. J. 31, 172–178 (2002).

    Article  CAS  Google Scholar 

  16. Klar, T. A., Jakobs, S., Dyba, M., Egner, A. & Hell, S. W. Proc. Natl Acad. Sci. USA 97, 8206–8210 (2000).

    Article  CAS  Google Scholar 

  17. Betzig, E. et al. Science 313, 1642–1645 (2006).

    Article  CAS  Google Scholar 

  18. Rust, M. J., Bates, M. & Zhuang, X. Nat. Methods 3, 793–795 (2006).

    Article  CAS  Google Scholar 

  19. Pyne, A., Thompson, R., Leung, C., Roy, D. & Hoogenboom, B. W. Small 10, 3257–3261 (2014).

    Article  CAS  Google Scholar 

  20. Scheuring, S. et al. EMBO J. 18, 4981–4987 (1999).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. London Centre for Nanotechnology, University College London, London, UK

    Bart W. Hoogenboom

  2. Department of Physics and Astronomy, University College London, London, UK

    Bart W. Hoogenboom

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  1. Bart W. Hoogenboom
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Correspondence to Bart W. Hoogenboom.

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Hoogenboom, B.W. Stretching the resolution limit of atomic force microscopy. Nat Struct Mol Biol 28, 629–630 (2021). https://doi.org/10.1038/s41594-021-00638-x

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