In the first comprehensive mRNA isoform atlas of the developing and adult mouse brain, we discover that region and age influence the isoform repertoire of cell subtypes. We link peak cell type regulation to the critical development period and report attenuated levels in adulthood.
This is a preview of subscription content, log in via an institution to check access.
References
Aldridge, S. & Teichmann, S. A. Single cell transcriptomics comes of age. Nat. Commun. 11, 4307 (2020). A review of single-cell transcriptomics.
Raj, B. & Blencowe, B. J. Alternative splicing in the mammalian nervous system: recent insights into mechanisms and functional roles. Neuron 87, 14–27 (2015). A review summarizing known roles of alternative splicing in the brain.
Joglekar, A. et al. A spatially resolved brain region- and cell type-specific isoform atlas of the postnatal mouse brain. Nat. Commun. 12, 463 (2021). A study of isoform diversity in the postnatal mouse brain.
Gupta, I. et al. Single-cell isoform RNA sequencing characterizes isoforms in thousands of cerebellar cells. Nat. Biotechnol. 36, 1197–1202 (2018). An article outlining a method to sequence full-length RNA isoforms from single cells.
Hardwick, S. A. et al. Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue. Nat. Biotechnol. 40, 1082–1092 (2022). A method to sequence full-length isoforms from frozen nuclei and the insights it unlocks about the human brain.
Prjibelski, A. D. et al. Accurate isoform discovery with IsoQuant using long reads. Nat. Biotechnol. 41, 915–918 (2023). A computational tool to reconstruct RNA isoforms from bulk and single-cell long-read data.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Joglekar, A. et al. Single-cell long-read sequencing-based mapping reveals specialized splicing patterns in developing and adult mouse and human brain. Nat. Neurosci. https://doi.org/10.1038/s41593-024-01616-4 (2024).
Rights and permissions
About this article
Cite this article
Widespread changes in alternative splicing in developing and adult mouse brain. Nat Neurosci (2024). https://doi.org/10.1038/s41593-024-01617-3
Published:
DOI: https://doi.org/10.1038/s41593-024-01617-3
- Springer Nature America, Inc.