We developed a two-pronged strategy to functionally probe the enormous repertoire of noncoding DNA within genomes. Our approach markedly improved signal-to-noise ratio and successfully intersected single-cell genomics with reporter assays. The result delivers a multiplex and highly quantitative readout of regulatory sequences’ activity in dynamic and multicellular systems.
References
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This is a summary of: Lalanne, J.-B. et al. Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters. Nat. Methods https://doi.org/10.1038/s41592-024-02260-3 (2024).
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Quantitative profiling of regulatory DNA activity at single-cell resolution. Nat Methods 21, 936–937 (2024). https://doi.org/10.1038/s41592-024-02261-2
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DOI: https://doi.org/10.1038/s41592-024-02261-2
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