New condenser aperture designs form square or rectangular beams that match the camera dimensions, which efficiently expands the data acquisition area in cryogenic electron microscopy.
This is a preview of subscription content, log in via an institution to check access.
References
Glaeser, R. M. Annu. Rev. Biophys. 48, 45–61 (2019).
Danev, R., Yanagisawa, H. & Kikkawa, M. Trends Biochem. Sci. 44, 837–848 (2019).
Weis, F. et al. Acta Crystallogr. D 76, 724–728 (2020).
Nakane, T. et al. Nature 587, 152–156 (2020).
Young, L. N. & Villa, E. Annu. Rev. Biophys. 52, 573–595 (2023).
Obr, M. et al. J. Struct. Biol. 214, 107852 (2022).
Brown, H. G., Smith, D., Wardle, B. C. & Hanssen, E. Nat. Methods https://doi.org/10.1038/s41592-024-02206-9 (2024).
Chua, E. Y. D. et al. Nat. Methods https://doi.org/10.1038/s41592-023-02161-x (2024).
Phan, S. T. et al. Serial reconstruction and montaging from large-field electron microscope tomograms. In 2009 Ann. Conf. Proc. IEEE Eng. Med. Biol. Sci., 5772–5776 (IEEE, 2009).
Peck, A. et al. J. Struct. Biol. 214, 107860 (2022).
Yang, J. E. et al. Nat. Methods 20, 1537–1543 (2023).
Eisenstein, F. et al. Nat. Methods 20, 131–138 (2023).
Acknowledgements
I thank Z. Yu and N. Spellmon from cryo-EM shared resource, Janelia Research Campus, HHMI, for the helpful comments and discussions.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The author declares no competing interests.
Rights and permissions
About this article
Cite this article
Zhao, X. Unlocking cryo-EM’s multishot potential with square or rectangular beams. Nat Methods 21, 555–557 (2024). https://doi.org/10.1038/s41592-024-02224-7
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41592-024-02224-7
- Springer Nature America, Inc.