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Creating diploid assemblies from Nanopore and Illumina reads with hypo-assembler

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Diploid assembly is a difficult task that requires several types of genomic sequencing data, including — but not limited to — HiFi reads and parental sequences. Hypo-assembler, an assembly algorithm, uses high quality solid k-mers extracted from Illumina data alongside Nanopore reads to produce a high-quality diploid assembly using only Nanopore and Illumina data.

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Fig. 1: Overview of the hypo-assembler algorithm.

References

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This is a summary of: Darian, J. C. et al. Constructing telomere-to-telomere diploid genome by polishing haploid nanopore-based assembly. Nat. Methods https://doi.org/10.1038/s41592-023-02141-1 (2024).

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Creating diploid assemblies from Nanopore and Illumina reads with hypo-assembler. Nat Methods 21, 560–561 (2024). https://doi.org/10.1038/s41592-023-02142-0

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  • DOI: https://doi.org/10.1038/s41592-023-02142-0

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