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Evaluating long-read RNA-sequencing analysis tools with in silico mixtures

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We conducted a comprehensive long-read RNA sequencing (RNA-seq) benchmarking experiment by combining spike-ins and in silico mixtures to establish a ground-truth dataset. We used long- and short-read RNA-seq technology to deeply sequence samples and compared the performance of a range of analysis tools on these data.

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Fig. 1: Overview of the experimental design.

References

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This is a summary of: Dong, X. et al. Benchmarking long-read RNA-sequencing analysis tools using in silico mixtures. Nat. Methods https://doi.org/10.1038/s41592-023-02026-3 (2023).

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Evaluating long-read RNA-sequencing analysis tools with in silico mixtures. Nat Methods 20, 1643–1644 (2023). https://doi.org/10.1038/s41592-023-02027-2

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