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scNanoHi-C uncovers single-cell high-order chromatin structures and gene regulation

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Leveraging nanopore long-read sequencing, scNanoHi-C identifies multiway interactions between enhancers and their target promoters within a single cell. Compared with short-read-based single-cell Hi-C or population-based multiway sequencing methods, scNanoHi-C offers new opportunities to investigate the heterogeneities of single-cell gene regulation networks mediated by high-order 3D chromatin structures.

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Fig. 1: scNanoHi-C identifies multiway enhancer–promoter interactions within individual cells.

References

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This is a summary of: Li, W. et al. scNanoHi-C: a single-cell long-read concatemer sequencing method to reveal high-order chromatin structures within individual cells. Nat. Methods https://doi.org/10.1038/s41592-023-01978-w (2023).

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scNanoHi-C uncovers single-cell high-order chromatin structures and gene regulation. Nat Methods 20, 1456–1457 (2023). https://doi.org/10.1038/s41592-023-01979-9

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  • DOI: https://doi.org/10.1038/s41592-023-01979-9

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