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A method to map single-cell lineages in the mouse brain by CRISPR-based barcoding

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We developed CREST (CRISPR editing-based lineage-specific tracing) to enable high-throughput mapping of single-cell lineages in any Cre lineage of interest in mice. In addition, we delineated a comprehensive lineage landscape of the developing mouse ventral midbrain, revealing novel differentiation trajectories and molecular programs underlying neural specification.

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Fig. 1: CREST reveals comprehensive lineage landscape in the developing mouse ventral midbrain.

References

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This is a summary of: Xie, L. et al. Comprehensive spatiotemporal mapping of single-cell lineages in developing mouse brain by CRISPR-based barcoding. Nat. Methods https://doi.org/10.1038/s41592-023-01947-3 (2023).

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A method to map single-cell lineages in the mouse brain by CRISPR-based barcoding. Nat Methods 20, 1139–1140 (2023). https://doi.org/10.1038/s41592-023-01948-2

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