Increasing evidence suggests that the spatial distribution of biomolecules within cells is a critical component in deciphering single-cell molecular heterogeneity. State-of-the-art single-cell MS imaging is uniquely capable of localizing biomolecules within cells, providing a dimension of information beyond what is currently available through in-depth omics investigations.
This is a preview of subscription content, log in via an institution to check access.
References
Thul, P. J. et al. Science 356, eaal3321 (2017).
Pareek, V., Tian, H., Winograd, N. & Benkovic, S. J. Science 368, 283–290 (2020).
Cheung, T. K. et al. Nat. Methods 18, 76–83 (2021).
Cong, Y. Z. et al. Chem. Sci. 12, 1001–1006 (2021).
Zhu, H. et al. Nat. Methods 18, 788–798 (2021).
Capolupo, L. et al. Science 376, eabh1623 (2022).
Keren, L. et al. Sci. Adv. 5, eaax5851 (2019).
Angelo, M. et al. Nat. Med. 20, 436–442 (2014).
Giesen, C. et al. Nat. Methods 11, 417–422 (2014).
Jackson, H. W. et al. Nature 578, 615–620 (2020).
Kuett, L. et al. Nat. Cancer 3, 122–133 (2022).
Bandura, D. R. et al. Anal. Chem. 81, 6813–6822 (2009).
Rendeiro, A. F. et al. Nature 593, 564–569 (2021).
Yagnik, G., Liu, Z., Rothschild, K. J. & Lim, M. J. J. Am. Soc. Mass Spectrom. 32, 977–988 (2021).
Passarelli, M. K. et al. Nat. Methods 14, 1175–1183 (2017).
Yuan, Z. et al. Nat. Methods 18, 1223–1232 (2021).
Kompauer, M., Heiles, S. & Spengler, B. Nat. Methods 14, 90–96 (2017).
Niehaus, M., Soltwisch, J., Belov, M. E. & Dreisewerd, K. Nat. Methods 16, 925–931 (2019).
Castro, D. C., Xie, Y. R., Rubakhin, S. S., Romanova, E. V. & Sweedler, J. V. Nat. Methods 18, 1233–1238 (2021).
Rappez, L. et al. Nat. Methods 18, 799–805 (2021).
Wang, H. A. O. et al. Anal. Chem. 85, 10107–10116 (2013).
Stolee, J. A. & Vertes, A. Anal. Chem. 85, 3592–3598 (2013).
Li, X. et al. J. Am. Chem. Soc. 143, 21648–21656 (2021).
Yin, R., Burnum-Johnson, K. E., Sun, X., Dey, S. K. & Laskin, J. Nat. Protoc. 14, 3445–3470 (2019).
Sparvero, L. J. et al. Angew. Chem. Int. Ed. 60, 11784–11788 (2021).
Kuznetsov, I. et al. Nat. Commun. 6, 6944 (2015).
Li, L., Garden, R. W. & Sweedler, J. V. Trends Biotechnol. 18, 151–160 (2000).
Li, L. et al. Anal. Chem. 72, 3867–3874 (2000).
Jurchen, J. C., Rubakhin, S. S. & Sweedler, J. V. J. Am. Soc. Mass Spectrom. 16, 1654–1659 (2005).
Zhang, H. et al. Chem. Sci. 12, 8115–8122 (2021).
Li, G. Y. et al. Nat. Commun. 10, 4697 (2019).
Spraggins, J. M. et al. Anal. Chem. 91, 14552–14560 (2019).
Neumann, E. K. et al. Anal. Chem. 92, 13084–13091 (2020).
Acknowledgements
Aspects of this work were supported in part by the NIH grants R01 DK071801, R01 AG078794, RF1AG052324, P01CA250972, and the United States Department of Agriculture (grant number 2018-67001-28266). Some of our mass spectrometers were acquired using the NIH shared instrument grants S10OD028473, S10RR029531 and S10OD025084. H.Z. thanks for funding support a Postdoctoral Career Development Award provided by the American Society for Mass Spectrometry (2022). L.L. acknowledges a Pancreas Cancer Pilot grant from the University of Wisconsin Carbone Cancer Center (233-AAI9632), a Diabetes Research Center pilot and feasibility grant by Washington University/University of Wisconsin-Madison (grant No. P30 DK020579), and a Vilas Distinguished Achievement Professorship and Charles Melbourne Johnson Distinguished Chair Professorship with funding provided by the Wisconsin Alumni Research Foundation and the University of Wisconsin-Madison School of Pharmacy.
Author information
Authors and Affiliations
Contributions
H.Z., D.G.D. and L.L. wrote the manuscript and L.L. provided funding support. All authors participated in the discussion and edited the manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interest.
Rights and permissions
About this article
Cite this article
Zhang, H., Delafield, D.G. & Li, L. Mass spectrometry imaging: the rise of spatially resolved single-cell omics. Nat Methods 20, 327–330 (2023). https://doi.org/10.1038/s41592-023-01774-6
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41592-023-01774-6
- Springer Nature America, Inc.
This article is cited by
-
Spatial multimodal analysis of transcriptomes and metabolomes in tissues
Nature Biotechnology (2023)
-
Evaluation of antibody-based single cell type imaging techniques coupled to multiplexed imaging of N-glycans and collagen peptides by matrix-assisted laser desorption/ionization mass spectrometry imaging
Analytical and Bioanalytical Chemistry (2023)
-
Chemical tagging mass spectrometry: an approach for single-cell omics
Analytical and Bioanalytical Chemistry (2023)