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Fast cryo-electron tomography data acquisition to determine molecular structures in situ

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To accelerate data acquisition for in situ cryo-electron tomography, we created a method that takes into consideration sample geometry for the robust prediction of sample movement while the microscope stage is tilted. This approach enabled the parallel collection of tens to hundreds of tilt series.

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Fig. 1: Parallel cryoET in situ.

References

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This is a summary of: Eisenstein, F. et al. Parallel cryo electron tomography on in situ lamellae. Nat. Methods https://doi.org/10.1038/s41592-022-01690-1 (2022)

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Fast cryo-electron tomography data acquisition to determine molecular structures in situ. Nat Methods 20, 42–43 (2023). https://doi.org/10.1038/s41592-022-01691-0

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  • DOI: https://doi.org/10.1038/s41592-022-01691-0

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