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Post-translational modification

Pyro(phospho)mania

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Understanding the role of pyrophosphorylation requires specific analytical strategies to discriminate it from protein phosphorylation. A custom workflow reveals that nucleolar protein pyrophosphorylation in human cells regulates the transcription of ribosomal DNA.

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Fig. 1: Tailored proteomics workflow for the identification of pyrophosphorylation reveals extensive IP6K-dependent nuclear and nucleolar modification.

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Authors and Affiliations

  1. Centre for Proteome Research, Institute of Systems, Molecular & Integrative Biology, University of Liverpool, Liverpool, UK

    Claire E. Eyers & Christopher J. Clarke

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  1. Claire E. Eyers
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  2. Christopher J. Clarke
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Correspondence to Claire E. Eyers.

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The authors declare no competing interests.

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Eyers, C.E., Clarke, C.J. Pyro(phospho)mania. Nat Chem Biol (2024). https://doi.org/10.1038/s41589-024-01606-4

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