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Cell metabolism

Reporting NADPH fluxes

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The NADP+/NADPH coenzyme couple powers cellular biosynthesis and oxidative defense. A new study tracing glucose-derived deuterium during proline biosynthesis analyzes subcellular perturbations in NADPH utilization, revealing that NADP+/NADPH coenzyme pools in the cytosol and mitochondria are regulated independently.

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Fig. 1: Compartment-specific NADPH fluxes detected using deuterium tracing.

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Authors and Affiliations

  1. Donald B. and Catherine C. Marron Cancer Metabolism Center, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    Justin R. Cross

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  1. Justin R. Cross
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Correspondence to Justin R. Cross.

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The author declares no competing interests.

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Cross, J.R. Reporting NADPH fluxes. Nat Chem Biol 19, 792–794 (2023). https://doi.org/10.1038/s41589-023-01298-2

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