RNA modifications are emerging regulators of development and disease. A metabolic labeling approach using 5-fluorocytidine (5-FCyd) allows the mechanism-based profiling of several RNA-modifying enzymes and potentially links their activity to novel RNA targets.
References
Saghatelian, A. & Cravatt, B. F. Nat. Chem. Biol. 1, 130–142 (2005).
Liu, Y., Patricelli, M. P. & Cravatt, B. F. Proc. Natl. Acad. Sci. USA 96, 14694–14699 (1999).
Dai, W. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-021-00874-8 (2021).
Wiener, D. & Schwartz, S. Nat. Rev. Genet. 22, 119–131 (2021).
Khoddami, V. & Cairns, B. R. Nat. Biotechnol. 31, 458–464 (2013).
Anders, L. et al. Nat. Biotechnol. 32, 92–96 (2014).
Bantscheff, M. et al. Nat. Biotechnol. 25, 1035–1044 (2007).
Montgomery, D. C., Sorum, A. W. & Meier, J. L. J. Am. Chem. Soc. 136, 8669–8676 (2014).
Selmi, T. et al. Nucleic Acids Res. 49, 1006–1022 (2021).
Marchand, V., Ayadi, L., Bourguignon-Igel, V., Helm, M. & Motorin, Y. Methods Mol. Biol. 2298, 77–95 (2021).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Thalalla Gamage, S., Meier, J.L. Activity-based profiling in RNA. Nat Chem Biol 17, 1121–1122 (2021). https://doi.org/10.1038/s41589-021-00879-3
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41589-021-00879-3
- Springer Nature America, Inc.
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.