A high-throughput reporter gene assay for use in living tissues has been developed. The assay allows investigators to quantify, in parallel, the activities of reporter genes in each of the cell types that constitute a tissue.
This is a preview of subscription content, log in via an institution to check access.
References
White, M. A. Understanding how cis-regulatory function is encoded in DNA sequence using massively parallel reporter assays and designed sequences. Genomics 106, 165–170 (2015). A review article that describes MPRAs and their use in studying CRSs.
Zhang, F. & Lupski, J. R. Non-coding genetic variants in human disease. Hum. Mol. Genet. 24, 102–110 (2015). A review on the role of non-coding variants in human disease.
Cao, J. et al. Comprehensive single-cell transcriptional profiling of a multicellular organism. Science 357, 661–667 (2017). An article that describes the use of combinatorial indexing to sequence the transcriptomes of the cells in Caenorhabditis elegans.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Zhao, S. et al. A single-cell massively parallel reporter assay detects cell-type-specific gene regulation Nat. Genet. https://doi.org/10.1038/s41588-022-01278-7 (2022).
Rights and permissions
About this article
Cite this article
A scalable technology for measuring cell-type-specific activity of cis-regulatory sequences. Nat Genet 55, 172–173 (2023). https://doi.org/10.1038/s41588-022-01277-8
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41588-022-01277-8
- Springer Nature America, Inc.