Cancer cells frequently amplify oncogenes on DNA molecules outside of chromosomes — extrachromosomal DNA. A technique adapted for isolation of extrachromosomal DNA, termed CRISPR-CATCH, enables analyses of its genetic and epigenetic compositions, which provides insights into its origin, structural diversity and mechanism of oncogene activation in cancer.
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References
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This is a summary of: Chang, H. Y. et al. Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH. Nat. Genet. https://doi.org/10.1038/s41588-022-01190-0 (2022).
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Profiling oncogenic extra-chromosomal DNA in cancer. Nat Genet 54, 1591–1592 (2022). https://doi.org/10.1038/s41588-022-01193-x
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DOI: https://doi.org/10.1038/s41588-022-01193-x
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