Skip to main content
SpringerLink
Log in

Exploring tRNAs and their modifications and crosstalk using Nano-tRNAseq

  • Research Briefing
  • Published:

From Nature Biotechnology

View current issue Submit your manuscript

Nano-tRNAseq is a nanopore-based, cost-effective and high-throughput approach to quantify transfer RNA (tRNA) abundances and modifications simultaneously, providing a framework to study the ‘tRNAome’ at single-molecule resolution. We envision that Nano-tRNAseq will enable us to study the role of tRNA molecules and their modifications in a wide variety of contexts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1: Nano-tRNAseq quantifies tRNA abundances and modifications.

References

  1. Torres, A. G., Batlle, E. & Ribas de Pouplana, L. Role of tRNA modifications in human diseases. Trends Mol. Med. 20, 306–314 (2014). A comprehensive review of the human tRNA modifications and their links to disease.

    Article  CAS  PubMed  Google Scholar 

  2. Goodarzi, H. et al. Modulated expression of specific tRNAs drives gene expression and cancer progression. Cell 165, 1416–1427 (2016). This work showed that specific tRNAs are upregulated in breast cancer cells with increased metastatic potential.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Zhang, W., Foo, M., Eren, A. M. & Pan, T. tRNA modification dynamics from individual organisms to metaepitranscriptomics of microbiomes. Mol. Cell 82, 891–906 (2022). A review of tRNA modification dynamics, NGS-based methods for tRNA quantification, and the potential of using tRNA modifications to study biological communities such as microbiomes.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Thomas, N. K. et al. Direct nanopore sequencing of individual full length tRNA strands. ACS Nano. 15, 16642–16653 (2021). This work used nanopore DRS to sequence native tRNA molecules.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Begik, O. et al. Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing. Nat. Biotechnol. 39, 1278–1291 (2021). This work shows that RNA modifications can be detected as base-calling errors in nanopore DRS, but that their signatures differ depending on the modification type and sequence context.

    Article  CAS  PubMed  Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Lucas, M. C. et al. Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01743-6 (2023).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Exploring tRNAs and their modifications and crosstalk using Nano-tRNAseq. Nat Biotechnol 42, 38–39 (2024). https://doi.org/10.1038/s41587-023-01755-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41587-023-01755-2

  • Springer Nature America, Inc.

Search

Navigation