CRISPR–Cas-associated transposases enable targeted integration of new sequences into genomes.
References
Pickar-Oliver, A. & Gersbach, C.A. Nat. Rev. Mol. Cell Biol. https://doi.org/10.1038/s41580-019-0131-5 (2019).
Strecker, J. et al. Science 365, 48–53 (2019).
Klompe, S.E., Vo, P.L.H., Halpin-Healy, T.S. & Sternberg, S.H. Nature https://doi.org/10.1038/s41586-019-1323-z (2019).
Deyle, D. R. & Russell, D. W. Curr. Opin. Mol. Ther. 11, 442–447 (2009).
Gaj, T., Sirk, S. J. & Barbas, C. F.3rd. Biotechnol. Bioeng. 111, 1–15 (2014).
Mills, R. E. et al. Trends Genet. 23, 183–191 (2007).
Peters, J. E., Makarova, K. S., Shmakov, S. & Koonin, E. V. Proc. Natl Acad. Sci. USA 114, E7358–E7366 (2017).
Gaj, T., Gersbach, C. A. & Barbas, C. F. 3rd Trends Biotechnol. 7, 397–405 (2013).
Kosicki, M., Tomberg, K. & Bradley, A. Nat. Biotechnol. 36, 765–771 (2018).
Schiroli, G. et al. Cell Stem Cell 24, 551–565 (2019).
Nelson, C. E. et al. Nat. Med. 25, 427–432 (2019).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Kwon, J.B., Gersbach, C.A. Jumping at the chance for precise DNA integration. Nat Biotechnol 37, 1004–1006 (2019). https://doi.org/10.1038/s41587-019-0210-3
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41587-019-0210-3
- Springer Nature America, Inc.
This article is cited by
-
Insight into the molecular mechanism of the transposon-encoded type I-F CRISPR-Cas system
Journal of Genetic Engineering and Biotechnology (2023)