Skip to main content
SpringerLink
Log in

IBEX: a user-friendly and open-source solution for high-plex immunostaining

  • Tools of the Trade
  • Published:

From Nature Reviews Immunology

View current issue Sign up to alerts

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Radtke, A. J. et al. IBEX: a versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proc. Natl Acad. Sci. USA 117, 33455–33465 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Radtke, A. J. et al. IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues. Nat. Protoc. 17, 378–401 (2022).

    Article  CAS  PubMed  Google Scholar 

  3. Gola, A. et al. Commensal-driven immune zonation of the liver promotes host defence. Nature 589, 131–136 (2021).

    Article  CAS  PubMed  Google Scholar 

  4. Li, W., Germain, R. N. & Gerner, M. Y. Multiplex, quantitative cellular analysis in large tissue volumes with clearing-enhanced 3D microscopy (Ce3D). Proc. Natl Acad. Sci. USA 114, E7321–E7330 (2017).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Li, W., Germain, R. N. & Gerner, M. Y. High-dimensional cell-level analysis of tissues with Ce3D multiplex volume imaging. Nat. Protoc. 14, 1708–1733 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    Leanne Arakkal

Authors
  1. Leanne Arakkal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Leanne Arakkal.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Arakkal, L. IBEX: a user-friendly and open-source solution for high-plex immunostaining. Nat Rev Immunol 23, 783 (2023). https://doi.org/10.1038/s41577-023-00963-w

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41577-023-00963-w

  • Springer Nature Limited

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Search

Navigation