Skip to main content
SpringerLink
Log in

Nanopore-sequencing-based method for rapid poliovirus detection during outbreaks

  • Research Briefing
  • Published:

From Nature Microbiology

View current issue Submit your manuscript

Detection of poliovirus by cell culture and subsequent serotype identification via Sanger sequencing can be slow, delaying responses to emerging outbreaks. Direct virus detection using nested reverse transcription PCR and nanopore sequencing was prospectively validated in the Democratic Republic of the Congo and yielded accurate results in a fraction of the time.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1: DDNS and standard algorithm for poliovirus detection.

References

  1. UNICEF Data Warehouse (UNICEF, 23 January 2023); https://go.nature.com/44H5sw6. Public health statistics for children summarized by UNICEF.

  2. Global Polio Surveillance Action Plan 2022–2024 (World Health Organization, 2022); https://go.nature.com/3RcsOqh. A report illustrating the challenges faced by polio eradication and the proposed solutions.

  3. Shaw, A. G. et al. Time taken to detect and respond to polio outbreaks in Africa and the potential impact of direct molecular detection and nanopore sequencing. J. Infect. Dis. 226, 453–462 (2022). An article that presents the logistical challenges of testing stool samples for polio in the African region.

    Article  CAS  PubMed  Google Scholar 

  4. Shaw, A. G. et al. Rapid and sensitive direct detection and identification of poliovirus from stool and environmental surveillance samples by use of nanopore sequencing. J. Clin. Microbiol. 58, e00920-20 (2020). An article that presents the DDNS method.

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Shaw, A. G. et al. Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study. Nat. Microbiol. https://doi.org/10.1038/s41564-023-01453-4 (2023).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nanopore-sequencing-based method for rapid poliovirus detection during outbreaks. Nat Microbiol 8, 1764–1765 (2023). https://doi.org/10.1038/s41564-023-01487-8

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41564-023-01487-8

  • Springer Nature Limited

Search

Navigation