CRISPR immunity begins with the acquisition of sequences from invading nucleic acids through spacer integration into a CRISPR locus. Off-target integration of spacers into other parts of the genome is now implicated as a spontaneous source of new CRISPR loci.
References
Mohanraju, P. et al. Science 353, aad5147 (2016).
Jackson, S. A. et al. Science 356, eaal5056 (2017).
Nivala, J., Shipman, S. L. & Church, G. M. Nat. Microbiol. https://doi.org/10.1038/s41564-017-0097-z (2018).
Nuñez, J. K., Bai, L., Harrington, L. B., Hinder, T. L. & Doudna, J. A. Mol. Cell 62, 824–833 (2016).
Shipman, S. L., Nivala, J., Macklis, J. D. & Church, G. M. Science 353, aaf1175 (2016).
Shipman, S. L., Nivala, J., Macklis, J. D. & Church, G. M. Nature 547, 345–349 (2017).
Nuñez, J. K., Harrington, L. B., Kranzusch, P. J., Engelman, A. N. & Doudna, J. A. Nature 519, 193–198 (2015).
Charpentier, E., Richter, H., van der Oost, J. & White, M. FEMS Microbiol. Rev. 39, 428–441 (2015).
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Edraki, A., Sontheimer, E.J. CRISPRs from scratch. Nat Microbiol 3, 261–262 (2018). https://doi.org/10.1038/s41564-018-0115-9
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DOI: https://doi.org/10.1038/s41564-018-0115-9
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