A non-radical proximity labelling platform — BAP-seq — is presented that uses subcellular-localized BS2 esterase to convert unreactive enol-based probes into highly reactive acid chlorides in situ to label nearby RNAs. When paired with click-handle-mediated enrichment and sequencing, this chemistry enables high-resolution spatial mapping of RNAs across subcellular compartments.
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This is a summary of: Pani, S. et al. Bioorthogonal masked acylating agents for proximity-dependent RNA labelling. Nat. Chem. https://doi.org/10.1038/s41557-024-01493-1 (2024).
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Masked acid chlorides for proximity labelling of RNA. Nat. Chem. 16, 682–683 (2024). https://doi.org/10.1038/s41557-024-01492-2
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DOI: https://doi.org/10.1038/s41557-024-01492-2
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