Skip to main content
SpringerLink
Log in

Reply to: On gene silencing by the X10-23 DNAzyme

  • Matters Arising
  • Published:

From Nature Chemistry

View current issue Submit your manuscript

The Original Article was published on 18 July 2022

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1: Evaluation of the secondary structure of KRAS RNA variant b (NCBI reference sequence NM_004985.5).

Data availability

No new data were included in the response.

References

  1. Taylor, A. I. & Holliger, P. In vivo gene silencing by the modified X10-23 DNAzyme. Nat. Chem. https://doi.org/10.1038/s41557-022-00990-5 (2022).

  2. Wang, Y., Nguyen, K., Spitale, R. C. & Chaput, J. C. A biologically stable DNAzyme that efficiently silences gene expression in cells. Nat. Chem. 13, 319–326 (2021).

    Article  CAS  Google Scholar 

  3. Nguyen, K., Wang, Y. J., England, W. E., Chaput, J. C. & Spitale, R. C. Allele-specific RNA knockdown with a biologically stable and catalytically efficient XNAzyme. J. Am. Chem. Soc. 143, 4519–4523 (2021).

    Article  CAS  Google Scholar 

  4. Young, D. D., Lively, M. O. & Deiters, A. Activation and deactivation of DNAzyme and antisense function with light for the photochemical regulation of gene expression in mammalian cells. J. Am. Chem. Soc. 132, 6183–6193 (2010).

    Article  CAS  Google Scholar 

  5. Rivory, L. et al. The DNAzymes Rs6, Dz13 and DzF have potent biologic effects independent of catalytic activity. Oligonucleotides 16, 297–312 (2006).

    Article  CAS  Google Scholar 

  6. Takahashi, M. et al. Dual mechanisms of action of self-delivering, anti-HIV-1 FANA oligonucleotides as a potential new approach to HIV therapy. Mol. Ther. Nucleic Acids 17, 615–625 (2019).

    Article  CAS  Google Scholar 

  7. Crooke, S. T. Antisense Drug Technology: Principles, Strategies and Applications 2nd edn (CRC Press, 2008).

    Google Scholar 

Download references

Acknowledgements

This work was supported by the W.M. Keck Foundation.

Author information

Authors and Affiliations

  1. Departments of Pharmaceutical Sciences, University of California, Irvine, CA, USA

    Robert C. Spitale & John C. Chaput

  2. Department of Chemistry, University of California, Irvine, CA, USA

    Robert C. Spitale & John C. Chaput

  3. Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, USA

    Robert C. Spitale & John C. Chaput

  4. Department of Chemical and Biomolecular Engineering, University of California, Irvine, CA, USA

    John C. Chaput

Authors
  1. Robert C. Spitale
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John C. Chaput
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

J.C.C. and R.C.S. wrote the response with input from K. Nguyen and Y. Wang. K.N. prepared the figure.

Corresponding author

Correspondence to John C. Chaput.

Ethics declarations

Competing interests

J.C.C. is a consultant for 1E Therapeutics. The work reported here occurred prior to this relationship. R.C.S. declares no competing interests.

Peer review

Peer review information

Nature Chemistry thanks Yingfu Li and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Spitale, R.C., Chaput, J.C. Reply to: On gene silencing by the X10-23 DNAzyme. Nat. Chem. 14, 859–861 (2022). https://doi.org/10.1038/s41557-022-00983-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41557-022-00983-4

  • Springer Nature Limited

This article is cited by

Search

Navigation