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GENOME EDITING

Base editing goes into hyperdrive

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CRISPR base editors can induce single-base-pair changes in the genome, although they are often inefficient. A study now shows that fusion of the DNA-binding domain of RAD51 to base editors enhances both the efficiency and the targeting range of optimized enzymes. These ‘hyper-editors’ offer effective tools for disease modeling and gene therapy.

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Fig. 1: Enhanced efficiency and expanded editing range of hyper-base-editing enzymes engineered to include a non-specific ssDNA-binding domain of the human RAD51 protein.

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Authors and Affiliations

  1. Sandra and Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA

    Alyna Katti & Lukas E. Dow

  2. Graduate School of Medical Sciences, Weill Cornell Medicine, New York, NY, USA

    Alyna Katti & Lukas E. Dow

  3. Department of Medicine, Weill Cornell Medicine, New York, NY, USA

    Lukas E. Dow

Authors
  1. Alyna Katti
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  2. Lukas E. Dow
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Correspondence to Lukas E. Dow.

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Competing interests

L.E.D. is an inventor on a patent that describes base-editing enzymes with increased efficiency and editing range: patent application PCT/US2019/040358 (filed 2 July 2019), international publication number WO2020/033083 (publication date 13 February 2020).

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Katti, A., Dow, L.E. Base editing goes into hyperdrive. Nat Cell Biol 22, 617–618 (2020). https://doi.org/10.1038/s41556-020-0521-0

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