Skip to main content
Log in

Using extracellular vesicles in blood to diagnose paediatric tuberculosis

  • Research Briefing
  • Published:

From Nature Biomedical Engineering

View current issue Submit your manuscript

Multiplex detection of two interacting Mycobacterium tuberculosis biomarkers on the surface of circulating extracellular vesicles, using a nanoplasmon-enhanced immunoassay, improves the diagnosis of tuberculosis in immunosuppressed children living with HIV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1: Schematic of a NEI for diagnosing TB.

References

  1. Dodd, P. J. et al. The global burden of tuberculosis mortality in children: a mathematical modelling study. Lancet Glob. Health 5, e898–e906 (2017). This review discusses the challenges and importance of diagnosing paediatric TB.

    Google Scholar 

  2. Njuguna, I. N. et al. Urgent versus post-stabilisation antiretroviral treatment in hospitalised HIV-infected children in Kenya (PUSH): a randomised controlled trial. Lancet HIV 5, e12–e22 (2018). This report describes the diagnostically challenging cohort of paediatric patients with TB analysed in this study.

    Article  Google Scholar 

  3. Liu, C. et al. Quantification of circulating Mycobacterium tuberculosis antigen peptides allows rapid diagnosis of active disease and treatment monitoring. Proc. Natl Acad. Sci. USA 114, 3969–3974 (2017). This study indicates the potential of Mtb-derived serum biomarkers to diagnose TB, using a sophisticated immunoassay that is read by mass spectrometry.

    Article  CAS  Google Scholar 

  4. Gaur, R. L. et al. LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis. PLoS Pathog. 10, e1004376 (2014). This article indicates how the two biomarkers used in our assay interact to mediate Mtb virulence.

    Article  Google Scholar 

  5. Schorey, J. S., Cheng, Y. & McManus, W. R. Bacteria- and host-derived extracellular vesicles—two sides of the same coin? J. Cell Sci. 134, jcs256628 (2021). This review reports that EVs from Mtb-infected macrophages can carry Mtb-derived factors and enter the circulatory system.

    Article  CAS  Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Zheng, W. et al. Diagnosis of paediatric tuberculosis by optically detecting two virulence factors on extracellular vesicles in blood samples. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-022-00922-1 (2022).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Using extracellular vesicles in blood to diagnose paediatric tuberculosis. Nat. Biomed. Eng 6, 930–931 (2022). https://doi.org/10.1038/s41551-022-00933-y

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41551-022-00933-y

  • Springer Nature Limited

Navigation