Skip to main content
Log in

GENOME EDITING

Enhanced precision and efficiency

  • News & Views
  • Published:

From Nature Biomedical Engineering

View current issue Submit your manuscript

The expression of two DNA repair factors improves the recombination of single-stranded oligodeoxynucleotides with Cas9-induced double-strand breaks, facilitating precise and efficient gene editing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1: Schematic of the DSB-repair pathways used in Cas9-induced genome editing.

References

  1. Barrangou, R. & Doudna, J. A. Nat. Biotechnol. 34, 933–941 (2016).

    Article  CAS  PubMed  Google Scholar 

  2. Daley, J. M. & Sung, P. Mol. Cell Biol. 34, 1380–1388 (2014).

    Article  PubMed  PubMed Central  Google Scholar 

  3. Heyer, W.-D., Ehmsen, K. T. & Liu, J. Annu. Rev. Genet. 44, 113–139 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Chen, F. et al. Nat. Methods 8, 753–755 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Richardson, C. D., Ray, G. J., DeWitt, M. A., Curie, G. L. & Corn, J. E. Nat. Biotechnol. 34, 339–344 (2016).

    Article  CAS  PubMed  Google Scholar 

  6. Liang, X., Potter, J., Kumar, S., Ravinder, N. & Chesnut, J. D. J. Biotechnol. 241, 136–146 (2017).

    Article  CAS  PubMed  Google Scholar 

  7. Renaud, J.-B. et al. Cell Rep. 14, 2263–2272 (2016).

    Article  CAS  PubMed  Google Scholar 

  8. Paulsen, B. S. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-017-0145-2 (2017).

  9. Zimmermann, M. & de Lange, T. Trends Cell Biol. 24, 108–117 (2014).

    Article  CAS  PubMed  Google Scholar 

  10. Hanamshet, K., Mazina, O. M. & Mazin, A. V. Genes 7, 63 (2016).

  11. Maeder, M. L. & Gersbach, C. A. Mol. Ther. J. Am. Soc. Gene Ther. 24, 430–446 (2016).

    Article  CAS  Google Scholar 

  12. Dever, D. P. et al. Nature 539, 384–389 (2016).

    Article  CAS  PubMed  Google Scholar 

  13. Richardson, C. D. et al. Preprint at http://dx.doi.org/10.1101/136028 (2017).

  14. Ceccaldi, R., Sarangi, P. & D’Andrea, A. D. Nat. Rev. Mol. Cell Biol. 17, 337–349 (2016).

    Article  CAS  PubMed  Google Scholar 

  15. Ma, M. et al. Cell Res. 27, 578–581 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ralf Kühn.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bashir, S., Kühn, R. Enhanced precision and efficiency. Nat Biomed Eng 1, 856–857 (2017). https://doi.org/10.1038/s41551-017-0159-9

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41551-017-0159-9

  • Springer Nature Limited

Navigation