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Gene delivery: Suddenly squeezed and shocked

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A microfluidic device that integrates mechanical squeezing and electrical stimulation delivers DNA to the nucleus of cells at a rate of millions of cells per minute.

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Figure 1: Schematic of the delivery of large molecules, such as DNA, into the nucleus of cells through mechanical squeezing of the cell (which disrupts the plasma membrane), followed by electric-field disruption of the nuclear membrane.

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Authors and Affiliations

  1. Mehmet Toner is at the Center for Engineering in Medicine and BioMicroElectroMechanical Systems Resource Center, Massachusetts General Hospital and Harvard Medical School, 114 16th Street, Charlestown, 02129, Massachusetts, USA

    Mehmet Toner

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  1. Mehmet Toner
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Correspondence to Mehmet Toner.

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Toner, M. Gene delivery: Suddenly squeezed and shocked. Nat Biomed Eng 1, 0047 (2017). https://doi.org/10.1038/s41551-017-0047

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  • DOI: https://doi.org/10.1038/s41551-017-0047

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