Skip to main content
SpringerLink
Log in

Molecular engineering

The power of repetition

  • News & Views
  • Published:

From Nature Plants

View current issue Submit your manuscript

The inclusion of retrotransposon long terminal repeats — and of other repeated sequences — enhances transfer DNA copy numbers in plant cells during transformation. Gene editing and homologous recombination-mediated gene targeting can therefore be improved by these means: however, the mechanism remains a mystery.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1: An alternative model for repeat-induced DNA amplification.

References

  1. Dickinson, L. et al. Nat. Plants https://doi.org/10.1038/s41477-023-01495-w (2023).

    Article  PubMed  Google Scholar 

  2. Gelvin, S. B. Annu. Rev. Genet. 51, 195–217 (2017).

    Article  CAS  PubMed  Google Scholar 

  3. Kralemann, L. E. M. et al. Nat. Plants 8, 526–534 (2022).

    Article  CAS  PubMed  Google Scholar 

  4. Puchta, H., Dujon, B. & Hohn, B. Proc. Natl Acad. Sci. USA 93, 5055–5060 (1996).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Dong, O. X. & Ronald, P. C. Proc. Natl Acad. Sci. USA 118, e2004834117 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Baltes, N. J., Gil-Humanes, J., Cermak, T., Atkins, P. A. & Voytas, D. F. Plant Cell 26, 151–163 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Wolter, F., Klemm, J. & Puchta, H. Plant J 94, 735–746 (2018).

    Article  CAS  PubMed  Google Scholar 

  8. Bakkeren, G., Koukolíková-Nicola, Z., Grimsley, N. & Hohn, B. Cell 57, 847–857 (1989).

    Article  CAS  PubMed  Google Scholar 

  9. Peng, H., Mirouze, M. & Bucher, E. Curr. Opin. Plant Biol. 69, 102263 (2022).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Joseph Gottlieb Kölreuter Institute for Plant Sciences (JKIP), Karlsruhe Institute of Technology, Karlsruhe, Germany

    Holger Puchta

Authors
  1. Holger Puchta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Holger Puchta.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Puchta, H. The power of repetition. Nat. Plants 9, 1377–1378 (2023). https://doi.org/10.1038/s41477-023-01496-9

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41477-023-01496-9

  • Springer Nature Limited

Search

Navigation