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BASE EDITING

Off-target effects and the solution

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Adenine base editors (ABEs) and cytosine base editors (CBEs) are powerful tools for targeted base conversions, but their editing specificities are largely unknown. Now, two reports demonstrate that ABEs are highly specific, but BE3-type CBEs tend to generate genome-wide off-target single nucleotide variations.

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Fig. 1: Targeting specificity of base editors.

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China

    Jintao Tang, Letian Chen & Yao-Guang Liu

  2. Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University, Guangzhou, China

    Jintao Tang, Letian Chen & Yao-Guang Liu

  3. College of Life Sciences, South China Agricultural University, Guangzhou, China

    Jintao Tang, Letian Chen & Yao-Guang Liu

Authors
  1. Jintao Tang
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  2. Letian Chen
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  3. Yao-Guang Liu
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Correspondence to Yao-Guang Liu.

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Tang, J., Chen, L. & Liu, YG. Off-target effects and the solution. Nat. Plants 5, 341–342 (2019). https://doi.org/10.1038/s41477-019-0406-z

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