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Gene conversion following CRISPR/Cas9 DNA cleavage: an overlooked effect

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References

  1. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337:816–21.

    Article  CAS  Google Scholar 

  2. Stark JM, Pierce AJ, Oh J, Pastink A, Jasin M. Genetic steps of mammalian homologous repair with distinct mutagenic consequences. Mol Cell Biol. 2004;24:9305–16.

    Article  CAS  Google Scholar 

  3. Ma H, Marti-Gutierrez N, Park SW, Wu J, Lee Y, K. Suzuki A, et al. Correction of a pathogenic gene mutation in human embryos. Nature. 2017;548:413–9.

    Article  CAS  Google Scholar 

  4. Adikusuma F, Piltz S, Corbett MA, Turvey M, McColl SR, Helbig KJ, R M, et al. Large deletions induced by Cas9 cleavage. Nature. 2018;560:E8–9.

    Article  CAS  Google Scholar 

  5. Egli D, Zuccaro MV, Kosicki M, Church GM, Bradley A, Jasin M. Inter-homologue repair in fertilized human eggs? Nature. 2018;560:E5–7.

    Article  CAS  Google Scholar 

  6. Javidi-Parsijani P, Lyu P, Makani V, Sarhan WM, Yoo KW, El-Korashi L, et al. CRISPR/Cas9 increases mitotic gene conversion in human cells. Gene Ther. 2020. https://doi.org/10.1038/s41434-020-0126-z.

  7. Papadakis MN, Patrinos GP. Contribution of gene conversion in the evolution of the human beta-like globin gene family. Hum Genet. 1999;104:117–25.

    Article  CAS  Google Scholar 

  8. Katic I, Grosshans H. Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans. Genetics. 2013;195:1173–6.

    Article  CAS  Google Scholar 

  9. Wilde JJ, Aida T, Wienish M, Zhang Q, Qi P, Feng G. Efficient zygotic genome editing via RAD51-enahnced interhomolog repair. BioRxiv 2018;263699. https://doi.org/10.1101/263699.

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Funding

GB receives funding from the National collaborative Research Infrastructure (NCRIS) via the Australian Phenomics Network, National Health and Medical Research Council of Australia (APP1143008), the Australian Research Council (DP180101494), and the National Natural Science Foundation of China (81772214).

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Correspondence to Gaetan Burgio.

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Burgio, G. Gene conversion following CRISPR/Cas9 DNA cleavage: an overlooked effect. Gene Ther 27, 245–246 (2020). https://doi.org/10.1038/s41434-020-0154-8

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